Spertzel R O, Hilmas D E, Brown J R, Mason D W
Infect Immun. 1975 Mar;11(3):481-7. doi: 10.1128/iai.11.3.481-487.1975.
Studies were conducted to determine the effects of sublethal, acute, total-body irradiation (600 R) on the immune response of mice to a replicating antigen. Irradiation was performed at varying times (0 to 21 days) prior to immunization with attenuated Venezuelan equine encephalomyelitis virus, TC-83. Development of protective immunity was studied by inoculating subgroups of irradiated mice with virulent Venezuelan equine encephalomyelitis virus on days 1 through 28 after immunization. Irradiation failed to affect the overt clinical response of mice inoculated with the attenuated strain, but antibody responses and the onset of protective immunity was delayed, particularly in mice irradiated 2 h to 3 days prior to vaccination. Immunity afforded by TC-83 developed more rapidly as the interval between irradiation and vaccination increased: when this interval was 14 or more days, the temporal course of immune response in irradiated mice was similar to that of nonirradiated vaccines. Persistence of TC-83 viremia was greatly prolonged in irradiated mice through the irradiation recovery period and probably provided the antigenic stimulus responsible for delayed development of effective immunity.
开展了多项研究，以确定亚致死剂量的全身急性照射（600拉德）对小鼠针对复制性抗原的免疫反应的影响。在用减毒的委内瑞拉马脑炎病毒TC-83进行免疫接种之前的不同时间点（0至21天）进行照射。通过在免疫接种后的第1天至第28天给受照射小鼠的亚组接种强毒委内瑞拉马脑炎病毒，研究保护性免疫的发展情况。照射并未影响接种减毒株小鼠的明显临床反应，但抗体反应和保护性免疫的开始出现延迟，尤其是在接种疫苗前2小时至3天接受照射的小鼠中。随着照射与接种疫苗之间的间隔增加，TC-83所提供的免疫力发展得更快：当此间隔为14天或更长时间时，受照射小鼠的免疫反应时间进程与未受照射的疫苗相似。在整个照射恢复期，受照射小鼠中TC-83病毒血症的持续时间大大延长，这可能提供了导致有效免疫发展延迟的抗原刺激。
