Choi Eun-Mi, Kim Ah-Jin, Kim Yeon-O, Hwang Jae-Kwan
Department of Biotechnology & Bioproducts Research Center, Yonsei University, Seoul, South Korea.
J Med Food. 2005 Winter;8(4):446-53. doi: 10.1089/jmf.2005.8.446.
Many polysaccharides obtained from natural sources are considered to be biological response modifiers and have been shown to enhance various immune responses. Here, we investigated the immunomodulating effects of arabinogalactan (AG) and fucoidan (FU) in vitro. Mouse spleen lymphocytes became cytotoxic to tumor cells after culture with AG and FU at concentrations of 10-100 microg/mL. Also, AG and FU were mitogenic in spleen lymphocytes and peripheral macrophages. Macrophages treated with AG and FU (10-100 microg/mL) exhibited induced tumoricidal activity and increased phagocytosis, lysosomal enzyme activity, and production of nitrite, H2O2, tumor necrosis factor (TNF)-alpha, and interleukin (IL)-6. However, AG and FU had little effect on the level of IL-1beta. Thus, the tumoricidal effect of AG- and FU-activated macrophages appeared to be mainly mediated by production of free radicals (NO and H2O2) and cytokines (TNF-alpha and IL-6). These data suggest that AG and FU are activators of lymphocytes and macrophages. This property may contribute to their effectiveness in the immunoprevention of cancer.
许多从天然来源获得的多糖被认为是生物反应调节剂，并已显示出能增强各种免疫反应。在此，我们在体外研究了阿拉伯半乳聚糖（AG）和岩藻依聚糖（FU）的免疫调节作用。小鼠脾淋巴细胞在与浓度为10 - 100微克/毫升的AG和FU培养后，对肿瘤细胞产生细胞毒性。此外，AG和FU在脾淋巴细胞和外周巨噬细胞中具有促有丝分裂作用。用AG和FU（10 - 100微克/毫升）处理的巨噬细胞表现出诱导的杀肿瘤活性，并增加了吞噬作用、溶酶体酶活性以及亚硝酸盐、过氧化氢、肿瘤坏死因子（TNF）-α和白细胞介素（IL）-6的产生。然而，AG和FU对IL - 1β水平影响很小。因此，AG和FU激活的巨噬细胞的杀肿瘤作用似乎主要由自由基（NO和H2O2）和细胞因子（TNF -α和IL - 6）的产生介导。这些数据表明AG和FU是淋巴细胞和巨噬细胞的激活剂。这一特性可能有助于它们在癌症免疫预防中的有效性。
