Iwalewa E O, Adewunmi C O, Omisore N O A, Adebanji O A, Azike C K, Adigun A O, Adesina O A, Olowoyo O G
Department of Pharmacology, Faculty of Pharmacy, Obafemi Awolowo University, Ile-Ife, Nigeria.
J Med Food. 2005 Winter;8(4):539-44. doi: 10.1089/jmf.2005.8.539.
Antioxidant and cytoprotective activities of boiled, cold, and methanolic extracts of nine edible vegetables in Southwest Nigeria were evaluated in the 1,1-diphenyl-2-picrylhydrazyl free radical assay and hemagglutination assay in bovine erythrocytes, respectively. Crassocephalum rubens showed the highest antioxidant activity (56.5%), Solanum americanum and Vernonia amygdalina exhibited moderate antioxidant activity (26.0-37.5% and 14.8-36.2%, respectively), Solanum macrocarpon, Telfaria occidentalis, Amaranthus hybridus, and Jatropha tanjorensis produced weak activity (1.6-15.8%, 1.6-7.7%, 2.8-6.62%, and 10.7-12.1%, respectively), while Celosia argentea and Talinum triangulare were pro-oxidants. It was also shown that extracts from all the vegetables are pro-oxidants at high concentrations of either 1 or 5 mg/mL or both. On the other hand, the studies on the cytoprotective effect showed that all the plant extracts demonstrated a very low hemagglutination titer value between 0.32 and 5.56 except S. americanum methanolic extract, which had a titer of 50.0. These results indicated correlation between the antioxidant properties and the hemagglutination values of these plant extracts; however, the membrane stabilizing capacity of the extracts supports the plants' antioxidant activity.
分别通过1,1-二苯基-2-苦基肼自由基测定法和牛红细胞血凝试验，对尼日利亚西南部9种可食用蔬菜的水煮提取物、冷提取物和甲醇提取物的抗氧化及细胞保护活性进行了评估。红凤菜表现出最高的抗氧化活性（56.5%），龙葵和扁桃斑鸠菊表现出中等抗氧化活性（分别为26.0 - 37.5%和14.8 - 36.2%），大果茄、西方葫芦、杂种苋和坦焦麻风树产生较弱活性（分别为1.6 - 15.8%、1.6 - 7.7%、2.8 - 6.62%和10.7 - 12.1%），而青葙和三角叶土人参是促氧化剂。研究还表明，所有蔬菜提取物在1mg/mL或5mg/mL或两者的高浓度下均为促氧化剂。另一方面，细胞保护作用研究表明，除龙葵甲醇提取物的效价为50.0外，所有植物提取物的血凝效价值均非常低，介于0.32和5.56之间。这些结果表明这些植物提取物的抗氧化特性与血凝值之间存在相关性；然而，提取物的膜稳定能力支持了植物的抗氧化活性。
