Siglienti Ines, Bendszus Martin, Kleinschnitz Christoph, Stoll Guido
Department of Neurology, Julius-Maximilians-Universität, Josef-Schneider-Str. 11, 97080 Würzburg, Germany.
J Neuroimmunol. 2006 Apr;173(1-2):166-73. doi: 10.1016/j.jneuroim.2005.11.011. Epub 2005 Dec 27.
Superparamagnetic iron oxide (SPIO/USPIO) particles are a promising new tool to label cells for in vivo monitoring of their migration into the nervous system by magnetic resonance imaging (MRI). Upon systemic application, SPIO/USPIO particles are preferentially internalized by macrophages. It is unclear whether this affects their immunological profile. We tested the cytokine production of rat and mouse macrophages in vitro and found that internalization of SPIO/USPIO shifted macrophages towards an anti-inflammatory, less responsive phenotype by enhancing interleukin (IL)-10 and inhibiting tumor necrosis factor (TNF)-alpha production. During macrophage interaction with T-cells IL-12p40 secretion was inhibited. Based on our in vitro findings, potential immunomodulatory effects of SPIO/USPIO particles in vivo warrant further investigation.
超顺磁性氧化铁（SPIO/USPIO）颗粒是一种很有前景的新型工具，可用于标记细胞，以便通过磁共振成像（MRI）在体内监测其向神经系统的迁移。全身应用后，SPIO/USPIO颗粒优先被巨噬细胞内化。目前尚不清楚这是否会影响其免疫特性。我们在体外测试了大鼠和小鼠巨噬细胞的细胞因子产生情况，发现SPIO/USPIO的内化通过增强白细胞介素（IL）-10和抑制肿瘤坏死因子（TNF）-α的产生，使巨噬细胞向抗炎、反应性较低的表型转变。在巨噬细胞与T细胞相互作用期间，IL-12p40的分泌受到抑制。基于我们的体外研究结果，SPIO/USPIO颗粒在体内的潜在免疫调节作用值得进一步研究。
