Kawagoshi Yasunori, Hino Naoe, Fujimoto Aya, Nakao Masaharu, Fujita Yukiko, Sugimura Seiji, Furukawa Kenji
Department of Civil and Environmental Engineering, Kumamoto University, 2-39-1 Kurokami, Japan.
J Biosci Bioeng. 2005 Nov;100(5):524-30. doi: 10.1263/jbb.100.524.
The effect of conditioning for a variety of inoculums on fermentative hydrogen production was investigated. In addition, the effects of pH condition on hydrogen fermentation and bacterial community were investigated. The effect of conditioning on hydrogen production was different depending on the inoculum types. An appreciable hydrogen production was shown with anaerobic digested sludge and lake sediment without conditioning, however, no hydrogen was produced when refuse compost and kiwi grove soil were used as inoculums without conditioning. The highest hydrogen production was obtained with heat-conditioned anaerobic digested sludge, almost the same production was also obtained with unconditioned digested sludge. The pH condition considerably affected hydrogen fermentation, hydrogen gas was efficiently produced with unconditioned anaerobic sludge when the pH was controlled at 6.0 throughout the culture period and not when only the initial pH was adjusted to 6.0 and 7.0. Hydrogen production decreased when the culture pH was only adjusted at the beginning of each batch in continuous batch culture, and additionally, bacterial community varied with the change in hydrogen production. It was suggested that Clostridium and Coprothermobacter species played important role in hydrogen fermentation, and Lactobacillus species had an adverse effect on hydrogen production.
研究了对多种接种物进行预处理对发酵产氢的影响。此外，还研究了pH条件对氢气发酵和细菌群落的影响。预处理对产氢的影响因接种物类型而异。未经预处理的厌氧消化污泥和湖泊沉积物显示出可观的产氢量，然而，当使用未经预处理的垃圾 compost 和猕猴桃果园土壤作为接种物时，未产生氢气。经热预处理的厌氧消化污泥产氢量最高，未经预处理的消化污泥产氢量也几乎相同。pH条件对氢气发酵有显著影响，当在整个培养期将pH控制在6.0时，未经预处理的厌氧污泥能高效产生氢气，而仅将初始pH调整为6.0和7.0时则不能。在连续分批培养中，仅在每批开始时调整培养pH，产氢量会降低，此外，细菌群落随产氢量的变化而变化。研究表明，梭菌属和嗜热栖热菌属在氢气发酵中起重要作用，而乳杆菌属对产氢有不利影响。
