Bru Roque, Sellés Susana, Casado-Vela Juan, Belchí-Navarro Sarai, Pedreño Maria Angeles
Departamento de Agroquímica y Bioquímica, Facultad de Ciencias, Universidad de Alicante, Apartado 99, E-03080 Alicante, Spain.
J Agric Food Chem. 2006 Jan 11;54(1):65-71. doi: 10.1021/jf051485j.
In grapevine (Vitis vinifera L.), defense responses after microbial infection or treatment with elicitors involve accumulation of phytoalexins, oxidative burst, and the synthesis of pathogenesis-related proteins. Oligosaccharide fractions from fungal or algal cell walls efficiently induce the defense responses, but a detailed analysis of the elicitor-plant cell surface interaction at the molecular level is precluded by the lack of chemically pure oligosaccharide elicitors. A grapevine liquid cell culture system was used to examine the properties of cyclodextrins (CDs) as inducers of defense responses. This work shows that the chemically pure heptakis(2,6-di-O-methyl)-betaCD caused a dramatic extracellular accumulation of the phytoalexin resveratrol and changes in peroxidase activity and isoenzymatic pattern. Other modified CDs tested on several grapevine cell lines resulted in different eliciting capacities of CDs and different sensibilities of the cell lines. The spent medium of elicited cultures was shown to disturb Botrytis cinerea growth in a plate assay.
在葡萄（Vitis vinifera L.）中，微生物感染或用激发子处理后的防御反应包括植保素积累、氧化爆发以及病程相关蛋白的合成。来自真菌或藻类细胞壁的寡糖组分能有效诱导防御反应，但由于缺乏化学纯的寡糖激发子，无法在分子水平上对激发子与植物细胞表面的相互作用进行详细分析。利用葡萄液体细胞培养系统研究了环糊精（CDs）作为防御反应诱导剂的特性。这项研究表明，化学纯的七（2,6-二-O-甲基）-β-环糊精会导致植保素白藜芦醇在细胞外大量积累，并引起过氧化物酶活性和同工酶模式的变化。在几种葡萄细胞系上测试的其他修饰环糊精导致了环糊精不同的诱导能力和细胞系不同的敏感性。在平板试验中，诱导培养的用过培养基显示出能干扰灰葡萄孢的生长。
