Stojanović Natasa, Krilov Dubravka, Herak Janko N
University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, Croatia.
Free Radic Res. 2006 Feb;40(2):135-40. doi: 10.1080/10715760500456789.
There is relatively little information on the role of high density lipoprotein (HDL) oxidation in atherogenesis although there are indications that oxidation might affect atheroprotective activities of HDL. Recently we reported the study on LDL oxidation initiated and sustained by traces of the transition metal ions under conditions, which favor slow oxidation. Here we report the results of the analogous study on the oxidation of the two HDL subclasses. The oxidation process was monitored by measuring the time dependence of oxygen consumption and concentration of the spin-trapped free radicals using EPR spectroscopy. In both HDL2 and HDL3 subclasses, the dependence of the oxidation process on the copper/lipoprotein molar ratio is different from that in LDL dispersions. Comparison of the kinetic profiles of HDL2 and HDL3 oxidation revealed that under all studied experimental conditions HDL2 was more susceptible to copper-induced oxidation than HDL3.
尽管有迹象表明氧化可能会影响高密度脂蛋白（HDL）的抗动脉粥样硬化活性，但关于HDL氧化在动脉粥样硬化形成中的作用的信息相对较少。最近我们报道了在有利于缓慢氧化的条件下，痕量过渡金属离子引发并维持低密度脂蛋白（LDL）氧化的研究。在此，我们报告关于两种HDL亚类氧化的类似研究结果。通过使用电子顺磁共振波谱（EPR）测量氧气消耗的时间依赖性以及自旋捕获自由基的浓度来监测氧化过程。在HDL2和HDL3亚类中，氧化过程对铜/脂蛋白摩尔比的依赖性与LDL分散体中的不同。HDL2和HDL3氧化动力学曲线的比较表明，在所有研究的实验条件下，HDL2比HDL3更容易受到铜诱导的氧化作用。
