Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, Orlando, FL, USA.
Methods Mol Biol. 2022;2419:213-246. doi: 10.1007/978-1-0716-1924-7_13.
The concept of lipid peroxidation has been known for a long time. It is now well established that LDL plays a major role in atherosclerosis. Oxidized low-density lipoprotein (Ox-LDL) has been studied for over 35 years. Numerous pro- and anti-atherogenic properties have been attributed to Ox-LDL. Component composition of Ox-LDL is complex due to the influence of various factors, including the source, method of preparation, storage and use. Hence, it is very difficult to clearly define and characterize Ox-LDL. It contains unoxidized and oxidized fatty acid derivatives both in the ester and free forms, their decomposition products, cholesterol and its oxidized products, proteins with oxidized amino acids and cross-links, polypeptides with varying extents of covalent modification with lipid oxidation products and many others. The measurement of lipid oxidation has been a great boon, not only to the understanding of the process but also in providing numerous serendipitous discoveries and methodologies. In this chapter, we outline the methodologies for the preparation and testing of various lipoproteins for oxidation studies.
脂质过氧化的概念由来已久。现在已经明确，LDL 在动脉粥样硬化中起着主要作用。氧化低密度脂蛋白 (Ox-LDL) 已经研究了超过 35 年。大量的促动脉粥样硬化和抗动脉粥样硬化特性归因于 Ox-LDL。由于各种因素的影响，包括来源、制备方法、储存和使用，Ox-LDL 的成分组成非常复杂。因此，很难清楚地定义和表征 Ox-LDL。它包含未氧化和氧化的脂肪酸衍生物，无论是在酯还是游离形式，其分解产物，胆固醇及其氧化产物，具有氧化氨基酸和交联的蛋白质，具有不同程度与脂质氧化产物共价修饰的多肽以及许多其他物质。脂质氧化的测量不仅对理解这一过程有很大的帮助，而且还提供了许多偶然的发现和方法。在这一章中，我们概述了用于氧化研究的各种脂蛋白的制备和测试方法。
