Yang Jin Ah, Park Dae Won, Sohn Jang Wook, Kim Min Ja
Graduate School of Life Science and Biotechnology, College of Medicine, Korea University, Seoul.
J Clin Microbiol. 2006 Jan;44(1):236-8. doi: 10.1128/JCM.44.1.236-238.2006.
We developed a novel PCR-restriction fragment length polymorphism test for the ccrB gene by using HinfI and BsmI for rapid typing of staphylococcal cassette chromosome mec (SCCmec). When tested with reference strains and methicillin-resistant Staphylococcus aureus isolates, the method proved to be valid and useful for rapid identification of four SCCmec types, especially type IV.
我们通过使用HinfI和BsmI开发了一种针对ccrB基因的新型聚合酶链反应-限制性片段长度多态性检测方法,用于葡萄球菌盒式染色体mec(SCCmec)的快速分型。当用参考菌株和耐甲氧西林金黄色葡萄球菌分离株进行测试时,该方法被证明对于快速鉴定四种SCCmec类型是有效且有用的,尤其是IV型。