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本文引用的文献

1
Methicillin-resistant Staphylococcus aureus disease in three communities.三个社区中的耐甲氧西林金黄色葡萄球菌疾病
N Engl J Med. 2005 Apr 7;352(14):1436-44. doi: 10.1056/NEJMoa043252.
2
A novel multiplex real-time PCR assay for rapid typing of major staphylococcal cassette chromosome mec elements.一种用于主要葡萄球菌盒式染色体mec元件快速分型的新型多重实时PCR检测方法。
J Clin Microbiol. 2004 Jul;42(7):3309-12. doi: 10.1128/JCM.42.7.3309-3312.2004.
3
Novel type V staphylococcal cassette chromosome mec driven by a novel cassette chromosome recombinase, ccrC.由新型盒式染色体重组酶ccrC驱动的新型V型葡萄球菌盒式染色体mec
Antimicrob Agents Chemother. 2004 Jul;48(7):2637-51. doi: 10.1128/AAC.48.7.2637-2651.2004.
4
Staphylococcal resistance revisited: community-acquired methicillin resistant Staphylococcus aureus--an emerging problem for the management of skin and soft tissue infections.葡萄球菌耐药性再探讨:社区获得性耐甲氧西林金黄色葡萄球菌——皮肤和软组织感染管理中一个新出现的问题。
Curr Opin Infect Dis. 2003 Apr;16(2):103-24. doi: 10.1097/00001432-200304000-00007.
5
Identification in methicillin-susceptible Staphylococcus hominis of an active primordial mobile genetic element for the staphylococcal cassette chromosome mec of methicillin-resistant Staphylococcus aureus.在对甲氧西林敏感的人葡萄球菌中鉴定出一种活跃的原始可移动遗传元件,该元件与耐甲氧西林金黄色葡萄球菌的葡萄球菌盒式染色体mec相关。
J Bacteriol. 2003 May;185(9):2711-22. doi: 10.1128/JB.185.9.2711-2722.2003.
6
Insights on antibiotic resistance of Staphylococcus aureus from its whole genome: genomic island SCC.从金黄色葡萄球菌全基因组看其抗生素耐药性:基因组岛SCC
Drug Resist Updat. 2003 Feb;6(1):41-52. doi: 10.1016/s1368-7646(03)00003-7.
7
Multiplex PCR strategy for rapid identification of structural types and variants of the mec element in methicillin-resistant Staphylococcus aureus.用于快速鉴定耐甲氧西林金黄色葡萄球菌中mec元件结构类型和变体的多重PCR策略
Antimicrob Agents Chemother. 2002 Jul;46(7):2155-61. doi: 10.1128/AAC.46.7.2155-2161.2002.
8
Novel type of staphylococcal cassette chromosome mec identified in community-acquired methicillin-resistant Staphylococcus aureus strains.在社区获得性耐甲氧西林金黄色葡萄球菌菌株中鉴定出新型葡萄球菌盒式染色体mec。
Antimicrob Agents Chemother. 2002 Apr;46(4):1147-52. doi: 10.1128/AAC.46.4.1147-1152.2002.
9
Population-based community prevalence of methicillin-resistant Staphylococcus aureus in the urban poor of San Francisco.旧金山城市贫困人口中耐甲氧西林金黄色葡萄球菌的基于人群的社区患病率。
Clin Infect Dis. 2002 Feb 15;34(4):425-33. doi: 10.1086/338069. Epub 2002 Jan 2.
10
The emergence and evolution of methicillin-resistant Staphylococcus aureus.耐甲氧西林金黄色葡萄球菌的出现与演变
Trends Microbiol. 2001 Oct;9(10):486-93. doi: 10.1016/s0966-842x(01)02175-8.

用于葡萄球菌盒式染色体mec元件快速分型的新型聚合酶链反应-限制性片段长度多态性分析

Novel PCR-restriction fragment length polymorphism analysis for rapid typing of staphylococcal cassette chromosome mec elements.

作者信息

Yang Jin Ah, Park Dae Won, Sohn Jang Wook, Kim Min Ja

机构信息

Graduate School of Life Science and Biotechnology, College of Medicine, Korea University, Seoul.

出版信息

J Clin Microbiol. 2006 Jan;44(1):236-8. doi: 10.1128/JCM.44.1.236-238.2006.

DOI:10.1128/JCM.44.1.236-238.2006
PMID:16390979
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1351960/
Abstract

We developed a novel PCR-restriction fragment length polymorphism test for the ccrB gene by using HinfI and BsmI for rapid typing of staphylococcal cassette chromosome mec (SCCmec). When tested with reference strains and methicillin-resistant Staphylococcus aureus isolates, the method proved to be valid and useful for rapid identification of four SCCmec types, especially type IV.

摘要

我们通过使用HinfI和BsmI开发了一种针对ccrB基因的新型聚合酶链反应-限制性片段长度多态性检测方法,用于葡萄球菌盒式染色体mec(SCCmec)的快速分型。当用参考菌株和耐甲氧西林金黄色葡萄球菌分离株进行测试时,该方法被证明对于快速鉴定四种SCCmec类型是有效且有用的,尤其是IV型。