Yu Bo, Xu Ping, Shi Quan, Ma Cuiqing
State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, People's Republic of China.
Appl Environ Microbiol. 2006 Jan;72(1):54-8. doi: 10.1128/AEM.72.1.54-58.2006.
The soil-isolated strain XP was identified as Rhodococcus erythropolis. R. erythropolis XP could efficiently desulfurize benzonaphthothiophene, a complicated model sulfur compound that exists in crude oil. The desulfurization product of benzonaphthothiophene was identified as alpha-hydroxy-beta-phenyl-naphthalene. Resting cells could desulfurize diesel oil (total organic sulfur, 259 ppm) after hydrodesulfurization. The sulfur content of diesel oil was reduced by 94.5% by using the resting cell biocatalyst for 24 h at 30 degrees C. Biodesulfurization of crude oils was also investigated. After 72 h of treatment at 30 degrees C, 62.3% of the total sulfur content in Fushun crude oil (initial total sulfur content, 3,210 ppm) and 47.2% of that in Sudanese crude oil (initial total sulfur, 1,237 ppm) were removed. Gas chromatography with pulsed-flame photometric detector analysis was used to evaluate the effect of R. erythropolis XP treatment on the sulfur content in Fushun crude oil, and it was shown that most organic sulfur compounds were eliminated after biodesulfurization.
从土壤中分离出的菌株XP被鉴定为红平红球菌。红平红球菌XP能够高效地对苯并萘噻吩进行脱硫，苯并萘噻吩是一种存在于原油中的复杂模型含硫化合物。苯并萘噻吩的脱硫产物被鉴定为α-羟基-β-苯基萘。静止细胞在加氢脱硫后能够对柴油（总有机硫含量为259 ppm）进行脱硫。在30℃下使用静止细胞生物催化剂24小时后，柴油中的硫含量降低了94.5%。还研究了原油的生物脱硫。在30℃下处理72小时后，抚顺原油（初始总硫含量为3210 ppm）中62.3%的总硫含量以及苏丹原油（初始总硫含量为1237 ppm）中47.2%的总硫含量被去除。使用带有脉冲火焰光度检测器的气相色谱分析法评估红平红球菌XP处理对抚顺原油中硫含量的影响，结果表明生物脱硫后大多数有机硫化合物被去除。
