感染水疱性口炎病毒的蚊细胞产生低感染性的非唾液酸化病毒粒子。
Mosquito cells infected with vesicular stomatitis virus yield unsialylated virions of low infectivity.
作者信息
Schloemer R H, Wagner R R
出版信息
J Virol. 1975 Apr;15(4):1029-32. doi: 10.1128/JVI.15.4.1029-1032.1975.
Abstract
Vesicular stomatitis virus propagated in and released from Aedes albopictus cells had the normal complement of viral proteins; the glycoprotein contained carbohydrate but no sialic acid. These virions had markedly reduced hemagglutinating activity and exhibited a very high ratio of physical particles to infectious virus. In vitro sialylation of vesicular stomatitis virions grown in mosquito cells resulted in a 100-fold increase in both infectivity and hemagglutination titers to levels approaching those of virus grown in BHK-21 cells. These experiments provide an example of host-controlled modification of viral infectivity.
摘要
在白纹伊蚊细胞中繁殖并释放的水疱性口炎病毒具有正常的病毒蛋白组成；糖蛋白含有碳水化合物但不含唾液酸。这些病毒粒子的血凝活性显著降低，并且物理粒子与感染性病毒的比例非常高。对在蚊细胞中生长的水疱性口炎病毒粒子进行体外唾液酸化处理后，其感染性和血凝效价均增加了100倍，达到接近在BHK - 21细胞中生长的病毒的水平。这些实验提供了一个宿主控制病毒感染性修饰的例子。
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