Keegstra K, Sefton B, Burke D
J Virol. 1975 Sep;16(3):613-20. doi: 10.1128/JVI.16.3.613-620.1975.
Sindbis virus was grown in four different host cells and the carbohydrate portions of the glycoproteins were analyzed. Sindbis virus grown in BHK-21 cells has more sialic acid and galactose than Sindbis virus grown in chicken embryo cells. In other respects the carbohydrates from virus grown in these two hosts are very similar. Sindbis virus grown either in chick cells transformed by Rous sarcoma virus or in BHK cells transformed by polyoma virus was also examined. In comparisons of virus from normal and transformed cells, differences in the amount of sialic acid were observed; but otherwise the carbohydrate structures appeared basically similar. The growth conditions used for the host cell also affected the degree of completion of the carbohydrate chains of the viral glycoproteins.
辛德毕斯病毒在四种不同的宿主细胞中培养,并对糖蛋白的碳水化合物部分进行了分析。在BHK - 21细胞中培养的辛德毕斯病毒比在鸡胚细胞中培养的辛德毕斯病毒含有更多的唾液酸和半乳糖。在其他方面,在这两种宿主中培养的病毒的碳水化合物非常相似。还检测了在劳斯肉瘤病毒转化的鸡细胞或多瘤病毒转化的BHK细胞中培养的辛德毕斯病毒。在比较正常细胞和转化细胞产生的病毒时,观察到唾液酸含量存在差异;但除此之外,碳水化合物结构基本相似。宿主细胞的培养条件也影响病毒糖蛋白碳水化合物链的完成程度。