Prather S O, Taylor M W
J Virol. 1975 Apr;15(4):872-81. doi: 10.1128/JVI.15.4.872-881.1975.
Restricted mengovirus replication in Mandin-Darby bovine kidney (MDBK) cells is characterized by a 400-fold reduction in infectious virus yield and a 40-fold increase in the production of noninfectious virus. Using conditions which insure that all MDBK cells are infected, virus-specific RNA and protein synthesis were measured in the restrictive host and in a permissive host for mengovirus, HeLa cells. Labeling kinetics and sucrose gradient analysis of mengovirus-specific RNA from MDBK cells show a reduction of 10-fold in virion RNA, 5-fold in double-stranded RNA, and 12.5-fold in single-stranded RNA. The viral RNA biosynthetic processes which occur late in the replicative cycle and result in the production of 90% of the single-stranded viral RNA that is packaged into capsid proteins in the permissive host are absent in restrictive MDBK cells. Viral protein synthesis as measured by labeled viral-specific polysome is decreased, and there is an accumulation of 80S subviral particles in the restricted host. It is suggested that restriction may act at a number of stages of viral replication and maturation.
在曼氏-达比牛肾（MDBK）细胞中，受限的脑心肌炎病毒复制的特征是感染性病毒产量降低400倍，非感染性病毒产量增加40倍。利用确保所有MDBK细胞均被感染的条件，在限制性宿主和脑心肌炎病毒的允许性宿主HeLa细胞中测量了病毒特异性RNA和蛋白质合成。对MDBK细胞中脑心肌炎病毒特异性RNA的标记动力学和蔗糖梯度分析显示，病毒粒子RNA减少10倍，双链RNA减少5倍，单链RNA减少12.5倍。在复制周期后期发生并导致在允许性宿主中90%被包装到衣壳蛋白中的单链病毒RNA产生的病毒RNA生物合成过程，在限制性MDBK细胞中不存在。通过标记的病毒特异性多核糖体测量的病毒蛋白质合成减少，并且在受限宿主中有80S亚病毒颗粒的积累。提示限制可能作用于病毒复制和成熟的多个阶段。
