Chinchar V G, Floyd A D, Chinchar G D, Taylor M W
Biochem Genet. 1979 Feb;17(1-2):133-48. doi: 10.1007/BF00484479.
Hypoxanthine-guanine phosphoribosyltransferase (HGPRT)-deficient mutants of a bovine kidney cell line (MDBK) were selected following mutagenesis with ethylmethane sulfonate or ICR-170G. MDBK mutants were hybridized to thymidine kinase-deficient L cells and selected in HAT medium. Parental and hybrid cells were characterized for isozyme patterns of lactic dehydrogenase malate dehydrogenase, glucose-6-phosphate dehydrogenase, and glutamate oxalate transaminase. Chromosomes of MDBK can be distinguished from mouse L cells by configuration and by fluorescent staining with Hoechst 33-258 stain. Hybrid cells contained both MDBK and L-cell chromosomes and had elevated DNA content. MDBK cells are normally restrictive for mengovirus replication. Both permissive and restrictive hybrids were found. Our data indicate that there was preferential loss of MDBK chromosomes in the hybrid cell lines.
用甲磺酸乙酯或ICR - 170G诱变后，筛选出牛肾细胞系（MDBK）的次黄嘌呤 - 鸟嘌呤磷酸核糖转移酶（HGPRT）缺陷型突变体。将MDBK突变体与胸苷激酶缺陷型L细胞杂交，并在HAT培养基中进行筛选。对亲代细胞和杂交细胞进行乳酸脱氢酶、苹果酸脱氢酶、葡萄糖 - 6 - 磷酸脱氢酶和谷氨酸草酰乙酸转氨酶同工酶模式的表征。MDBK的染色体可通过形态以及用Hoechst 33 - 258染色进行荧光染色与小鼠L细胞区分开来。杂交细胞同时含有MDBK和L细胞染色体，且DNA含量升高。MDBK细胞通常对脑心肌炎病毒复制具有限制性。发现了允许性和限制性杂交细胞。我们的数据表明，在杂交细胞系中MDBK染色体存在优先丢失现象。
