Wilkinson R, Kerr S J
J Virol. 1973 Nov;12(5):1013-9. doi: 10.1128/JVI.12.5.1013-1019.1973.
The tRNA methyltransferase activity in mengovirus-infected L cells, HeLa cells, and Maden Derby bovine kidney cells has been examined during the course of infection. The first two cell lines yield a productive infection, but have different kinetics of inhibition of host RNA synthesis, whereas the bovine kidney cells are a restrictive host. In infected L cells the enzymes show altered capacity and base specificity throughout the infection. In infected HeLa cells and in infected bovine kidney cells less marked changes were seen. No inhibitors or activators of the enzymes were detected in any of the infected cell lines. Labeling experiments in infected cells indicated that in infected L cells synthesis of RNA was inhibited to a greater degree than was methylation of RNA. The consequence of this would be a hypermethylation of RNA. The methylated derivatives synthesized in infected L cells showed changes in relative proportions. Infection of HeLa cells and bovine kidney cells did not show such marked effects on methylation of RNA.
在感染过程中，对脑心肌炎病毒感染的L细胞、HeLa细胞和马登-达比牛肾细胞中的tRNA甲基转移酶活性进行了检测。前两种细胞系会产生增殖性感染，但对宿主RNA合成的抑制动力学不同，而牛肾细胞是一种限制性宿主。在感染的L细胞中，整个感染过程中酶的活性和碱基特异性都发生了改变。在感染的HeLa细胞和感染的牛肾细胞中，观察到的变化不太明显。在任何感染的细胞系中均未检测到该酶的抑制剂或激活剂。感染细胞中的标记实验表明，在感染的L细胞中，RNA合成受到的抑制程度大于RNA甲基化受到的抑制程度。其结果将是RNA的超甲基化。在感染的L细胞中合成的甲基化衍生物的相对比例发生了变化。HeLa细胞和牛肾细胞的感染对RNA甲基化没有显示出如此明显的影响。
