Anderson R A, Burns T L, Wallace R B, Folsom A R, Sprafka J M
Iowa City VA Medical Center, University of Iowa College of Medicine.
Genet Epidemiol. 1992;9(2):109-21. doi: 10.1002/gepi.1370090204.
An explanation for the consistently documented finding of higher levels of high density lipoprotein-cholesterol in black men relative to white men was sought by comparing the frequency of restriction fragment length polymorphism markers present in blacks and in whites at the gene loci coding for the two major apolipoprotein constituents of high density lipoprotein, apolipoproteins AI and AII. The measurements were made in population-based samples of 45 to 54-year-old black (n = 190) and white (n = 370) subjects from the Minnesota Heart Survey for whom lipoprotein levels were available. The mean high density lipoprotein-cholesterol level for black men in the sample (47 +/- 1.5 mg/dl) was higher (P less than 0.05) than that for white men (42 +/- 0.9 mg/dl), while levels in women were not different between races. While the SacI and MspI markers at the apolipoprotein AI-CIII-AIV gene locus showed similar frequencies in blacks compared to whites, the degree of the linkage disequilibrium previously noted between these markers in white subjects was altered in blacks and the minor allele of the PstI marker at this locus was virtually absent in the black subjects (P less than 0.005 vs whites). For black men, there were significant associations of the M2 allele and the S2M2 haplotype at the apolipoprotein AI locus with lower high density lipoprotein-cholesterol levels. The results are consistent with the hypothesis that DNA sequence variations in the vicinity of the apolipoprotein AI-CIII-AIV gene locus are associated with the difference in high density lipoprotein-cholesterol levels between blacks and whites.
通过比较高密度脂蛋白的两种主要载脂蛋白成分即载脂蛋白AI和AII编码基因位点上黑人与白人中限制性片段长度多态性标记的出现频率，来探寻为何一直有文献记载黑人男性的高密度脂蛋白胆固醇水平高于白人男性。测量对象为明尼苏达心脏调查中45至54岁的黑人（n = 190）和白人（n = 370）基于人群的样本，这些样本的脂蛋白水平数据可得。样本中黑人男性的平均高密度脂蛋白胆固醇水平（47 +/- 1.5毫克/分升）高于白人男性（42 +/- 0.9毫克/分升）（P < 0.05），而不同种族女性的水平无差异。虽然载脂蛋白AI - CIII - AIV基因位点的SacI和MspI标记在黑人与白人中的出现频率相似，但之前在白人受试者中发现的这些标记之间的连锁不平衡程度在黑人中发生了改变，且该位点PstI标记的次要等位基因在黑人受试者中几乎不存在（与白人相比，P < 0.005）。对于黑人男性，载脂蛋白AI位点的M2等位基因和S2M2单倍型与较低的高密度脂蛋白胆固醇水平存在显著关联。这些结果与载脂蛋白AI - CIII - AIV基因位点附近的DNA序列变异与黑人和白人高密度脂蛋白胆固醇水平差异相关这一假设一致。
