Taleb Soraya, Cancello Raffaella, Poitou Christine, Rouault Christine, Sellam Philippe, Levy Patrick, Bouillot Jean-Luc, Coussieu Christiane, Basdevant Arnaud, Guerre-Millo Michèle, Lacasa Danièle, Clement Karine
Institut National de la Santé et de la Recherche Médicale U755, Nutrition Department, Hôtel Dieu, Place du parvis Notre Dame, 75004 Paris, France.
J Clin Endocrinol Metab. 2006 Mar;91(3):1042-7. doi: 10.1210/jc.2005-1601. Epub 2006 Jan 4.
Human adipose tissue produces several adipokines, including the newly identified protein cathepsin S (CTSS), a cysteine protease involved in the pathogenesis of atherosclerosis. Obesity is characterized by high levels of CTSS in the circulation and in sc white adipose tissue (scWAT).
We investigated the effect of surgery-induced weight loss on circulating CTSS and its protein expression in scWAT.
Fifty morbidly obese women before and 3 months after surgery and 10 healthy lean women were studied. We analyzed the relationships between circulating CTSS and clinical and biological parameters. Immunohistochemistry of the CTSS protein variations in scWAT was performed.
Weight loss decreased by 42% (P < 0.0001) the circulating CTSS levels, which correlated with changes in body weight (P = 0.03). We observed a significant decrease in CTSS enzymatic activity by 25% after weight loss (P = 0.001). Adipose tissue CTSS content was reduced by 30% (P = 0.002) after surgery. The variations in CTSS expression in scWAT after surgery correlated with changes in circulating CTSS serum levels (P = 0.03). Most of the correlations between CTSS and clinical and biological parameters disappeared after adjustment for body mass index, emphasizing the strong link between CTSS and corpulence in humans.
Changes in CTSS scWAT might contribute to serum variations in CTSS during weight loss. The decrease in CTSS concentrations in the circulation may contribute to vascular improvement in obese subjects after weight loss.
人体脂肪组织会产生多种脂肪因子,包括新发现的蛋白质组织蛋白酶S(CTSS),它是一种参与动脉粥样硬化发病机制的半胱氨酸蛋白酶。肥胖的特征是循环系统和皮下白色脂肪组织(scWAT)中CTSS水平升高。
我们研究了手术诱导的体重减轻对循环CTSS及其在scWAT中蛋白质表达的影响。
对50名病态肥胖女性在手术前和手术后3个月以及10名健康瘦女性进行了研究。我们分析了循环CTSS与临床和生物学参数之间的关系。对scWAT中CTSS蛋白质变化进行了免疫组织化学检测。
体重减轻使循环CTSS水平降低了42%(P < 0.0001),这与体重变化相关(P = 0.03)。我们观察到体重减轻后CTSS酶活性显著降低了25%(P = 0.001)。手术后脂肪组织CTSS含量降低了30%(P = 0.002)。手术后scWAT中CTSS表达的变化与循环CTSS血清水平的变化相关(P = 0.03)。在调整体重指数后,CTSS与临床和生物学参数之间的大多数相关性消失,强调了CTSS与人类肥胖之间的紧密联系。
CTSS在scWAT中的变化可能导致体重减轻期间CTSS的血清变化。循环中CTSS浓度的降低可能有助于肥胖受试者体重减轻后血管状况的改善。