大麦EST标记的染色体定位与缺失作图

Chromosomal assignment and deletion mapping of barley EST markers.

作者信息

Nasuda Shuhei, Kikkawa Yukari, Ashida Taizo, Islam A K M Rafiqul, Sato Kazuhiro, Endo Takashi R

机构信息

Laboratory of Plant Genetics, Graduate School of Agriculture, Kyoto University, Japan.

出版信息

Genes Genet Syst. 2005 Oct;80(5):357-66. doi: 10.1266/ggs.80.357.

DOI:10.1266/ggs.80.357

PMID:16394587

Abstract

From about 10000 PCR-based EST markers of barley we chose 1421 EST markers that were demonstrated to be amplified differently by PCR between wheat (Triticum aestivum cv. Chinese Spring) and barley (Hordeum vulgare cv. Betzes). We assigned them to the seven barley chromosomes (1H to 7H) by PCR analysis using a set of wheat-barley chromosome addition lines. We successfully assigned 701 (49.3%) EST markers to the barley chromosomes: 75 to 1H, 127 to 2H, 119 to 3H, 94 to 4H, 108 to 5H, 81 to 6H and 97 to 7H. By using a set of Betzes barley telosomic addition lines of Chinese Spring, we could successfully determine the chromosome-arm (S or L) location of at least 90% of the EST markers assigned to each barley chromosome. We conducted a trial mapping using 90 EST markers assigned to 7HS (49) or 7HL (41) and 19 wheat lines carrying 7H structural changes. More EST markers were found in the distal region than in the proximal region.

摘要

从大约10000个基于PCR的大麦EST标记中，我们选择了1421个EST标记，这些标记经PCR验证在小麦（普通小麦中国春品种）和大麦（大麦贝茨品种）之间扩增情况不同。我们利用一组小麦-大麦染色体附加系，通过PCR分析将它们定位到七条大麦染色体（1H至7H）上。我们成功地将701个（49.3%）EST标记定位到了大麦染色体上：75个定位到1H，127个定位到2H，119个定位到3H，94个定位到4H，108个定位到5H，81个定位到6H，97个定位到7H。通过使用一组中国春的大麦贝茨端体附加系，我们能够成功确定至少90%定位到每条大麦染色体上的EST标记的染色体臂（短臂或长臂）位置。我们使用90个定位到7HS（49个）或7HL（41个）的EST标记以及19个携带7H结构变化的小麦品系进行了初步定位。在远端区域发现的EST标记比近端区域更多。