Hoffmann G R, Malling H V
Mutat Res. 1975 Mar;27(3):307-18. doi: 10.1016/0027-5107(75)90287-0.
This paper reports the development of a new system for the detection of forward mutations in a heterokaryon of Neurospora crassa. This system, designed to detect a wide variety of genetic alterations, is based upon the detection of 8-azaguanine-resistant mutants in an adenine-requiring heterokaryon carrying the aza-3 gene. Induction of mutations in the aza-3 gene was detected after treatment of conidia with ultraviolet light, X-rays, ethyl methanesulfonate (EMS), or the acridine mustard, ICR-170. The pattern of appearance of mutant colonies and various factors in mutant selection are discussed.
本文报道了一种用于检测粗糙脉孢菌异核体中正向突变的新系统的开发。该系统旨在检测多种基因改变，它基于在携带aza - 3基因的腺嘌呤需求型异核体中检测对8 - 氮杂鸟嘌呤有抗性的突变体。在用紫外线、X射线、甲基磺酸乙酯（EMS）或吖啶芥子气ICR - 170处理分生孢子后，检测aza - 3基因中的突变诱导情况。讨论了突变菌落的出现模式以及突变体选择中的各种因素。
