利用对8-氮杂鸟嘌呤的抗性作为遗传标记，在鼠伤寒沙门氏菌中进行定量正向突变试验。
Quantitative forward mutation assay in Salmonella typhimurium using 8-azaguanine resistance as a genetic marker.
作者信息
Skopek T R, Liber H L, Krolewski J J, Thilly W G
出版信息
Proc Natl Acad Sci U S A. 1978 Jan;75(1):410-4. doi: 10.1073/pnas.75.1.410.
Abstract
We have developed a quantitative forward mutation assay using Salmonella typhimurium, in which resistance to the purine analog 8-azaguanine is used as a genetic marker. We present the assay protocol, the concentration-dependent toxicity and mutagenicity of five known mutagens (N-methyl-N'-nitro-N-nitrosoguanidine, ICR-191, 9-aminoacridine, dimethylnitrosamine, and benzo[a]pyrene), and reconstruction experiments testing the assay for possible bias. The relative merits of forward versus reverse mutation assays are discussed.
摘要
我们开发了一种利用鼠伤寒沙门氏菌的定量正向突变试验，其中对嘌呤类似物8-氮杂鸟嘌呤的抗性被用作遗传标记。我们展示了试验方案、五种已知诱变剂（N-甲基-N'-硝基-N-亚硝基胍、ICR-191、9-氨基吖啶、二甲基亚硝胺和苯并[a]芘）的浓度依赖性毒性和诱变性，以及测试该试验是否存在可能偏差的重建实验。还讨论了正向突变试验与反向突变试验的相对优点。
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Quantitative forward mutation assay in Salmonella typhimurium using 8-azaguanine resistance as a genetic marker.利用对8-氮杂鸟嘌呤的抗性作为遗传标记，在鼠伤寒沙门氏菌中进行定量正向突变试验。
Proc Natl Acad Sci U S A. 1978 Jan;75(1):410-4. doi: 10.1073/pnas.75.1.410.
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