Parsa Khankandi Hamed, Behzad Sahar, Mojab Faraz, Ahmadian-Attari Mohammad Mahdi, Sahranavard Shamim
Department of Pharmacognosy, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Evidence-based Phytotherapy and Complementary Medicine Research Center, Alborz University of Medical Sciences, Karaj, Iran.
Iran J Pharm Res. 2019 Spring;18(2):826-835. doi: 10.22037/ijpr.2019.1100685.
Nitric oxide (NO) is a key mediator that plays an important role in pathogenesis of various chronic diseases like Alzheimer's disease and Parkinson's disease. Additionally, there is a great attitude for finding natural compounds, which could control and inhibit NO production in pathological conditions. Therefore, we were encouraged to investigate the effects of some Lamiaceae species on NO production and cell injury during oxidative stress in PC12 cells. In this study, cell death determined by MTT assay and NO levels were evaluated using Griess assay. PC12 cells were exposed to total metanolic extracts of three and one species. The results revealed that () could protect PC12 cells from hydrogen proxide-induced oxidative stress and all of the plants inhibited NO production in that condition except (). In addition, () was meanwhile subjected to fractionation using different organic solvents. The dichloromethan and ethyl acetate fractions of could protect PC12 cells from oxidative stress injury. However, NO production was restrained by the hexane and dichloromethane fractions. Considering the results, , () and are good candidates for further investigations in neuroprotection and anti-inflammation studies.
一氧化氮（NO）是一种关键介质，在阿尔茨海默病和帕金森病等各种慢性疾病的发病机制中发挥着重要作用。此外，人们对寻找能够在病理条件下控制和抑制NO生成的天然化合物有着浓厚的兴趣。因此，我们受到鼓舞去研究一些唇形科植物物种对PC12细胞氧化应激期间NO生成和细胞损伤的影响。在本研究中，通过MTT法测定细胞死亡情况，并使用格里斯试剂法评估NO水平。PC12细胞暴露于三种和一种植物的总甲醇提取物中。结果显示，（）可以保护PC12细胞免受过氧化氢诱导的氧化应激，并且除了（）之外，所有植物在该条件下均抑制NO生成。此外，（）同时使用不同有机溶剂进行分离。该植物的二氯甲烷和乙酸乙酯馏分可以保护PC12细胞免受氧化应激损伤。然而，己烷和二氯甲烷馏分抑制了NO生成。考虑到这些结果，（）、（）和（）是神经保护和抗炎研究中进一步研究的良好候选对象。
