Kristensen Thomas, Baatrup Erik, Bayley Mark
Department of Zoophysiology, Institute of Biological Sciences, University of Aarhus, Building 135, DK-8000 Aarhus C, Denmark.
Ecotoxicol Environ Saf. 2006 Jan;63(1):148-57. doi: 10.1016/j.ecoenv.2005.03.010.
The ecologically highly important effect of intermale competition is assessed here in a new approach to evaluating endocrine disruption at the population level. A guppy (Poecilia reticulata) male exposed to the antiandrogenic DDT metabolite p,p'-DDE competed against an unexposed male for the opportunity to fertilize a female. Offspring were then assigned to sires using microsatellites in genetic paternity analysis. In addition, a suite of male sexual characteristics including sperm count, coloration, and sexual behavior were also measured. p,p'-DDE produced no significant harmful effect on either the male sexual characteristics or the success in siring young, although the highest sublethal dose tested was only a factor 10 below the dose producing 100% mortality. At present, data on the antiandrogenic potency of p,p'-DDE are highly ambiguous. This study supports the increasing amount of evidence that the demasculinizing action of this chemical is often weak and highly variable across populations, even within the same species.
本文采用一种评估种群水平内分泌干扰的新方法，评估了雄性间竞争在生态方面的重要影响。一只暴露于抗雄激素的滴滴涕代谢物p,p'-二氯二苯二氯乙烯（p,p'-DDE）的孔雀鱼（孔雀花鳉）雄性与一只未暴露的雄性竞争使雌性受精的机会。然后，在基因亲权分析中使用微卫星将后代归属于父本。此外，还测量了一系列雄性性特征，包括精子数量、体色和性行为。尽管测试的最高亚致死剂量仅比产生100%死亡率的剂量低10倍，但p,p'-DDE对雄性性特征或生育后代的成功率均未产生显著有害影响。目前，关于p,p'-DDE抗雄激素效力的数据非常模糊。这项研究支持了越来越多的证据，即这种化学物质的去雄作用通常较弱，并且在不同种群中变化很大，即使在同一物种内也是如此。
