Quinn Michael J, Summitt Cassie L, Ottinger Mary Ann
U.S. Army Center for Health Promotion and Preventive Medicine, Directorate of Toxicology, Health Effects Research Program, Edgewood Area, Bldg. E-2100, Aberdeen Proving Ground, MD 21010, USA.
Horm Behav. 2008 Jan;53(1):249-53. doi: 10.1016/j.yhbeh.2007.10.004. Epub 2007 Oct 12.
This study was conduced to assess the effects of a one time embryonic exposure to p,p'-DDE (dichlorodiphenyldichloroethylene; DDE) on the reproductive development and function in Japanese quail (Coturnix japonica). Embryos were exposed at day one of incubation to either 20 or 40 microg DDE or a sesame oil vehicle control (injection volume=20 microl). Onset of puberty, gonadal histopathology, sperm motility, cloacal gland size, and male copulatory behavior were assessed in adults. DDE accelerated onset of puberty in females and reduced male reproductive behaviors. Gonadal morphology and sperm motility appeared to be unaffected. Results from this study provide evidence that the neuroendocrine system may be more sensitive and less resilient to embryonic exposure to contaminants than traditional measures of reproductive success following contaminant exposure in adults. This study further supports the inclusion of behavioral assessments in toxicity tests.
本研究旨在评估一次性胚胎暴露于对，对'-滴滴伊（二氯二苯二氯乙烯；DDE）对日本鹌鹑（Coturnix japonica）生殖发育和功能的影响。在孵化第一天，将胚胎暴露于20或40微克DDE或芝麻油载体对照（注射体积=20微升）。对成年鹌鹑的青春期开始时间、性腺组织病理学、精子活力、泄殖腔腺大小和雄性交配行为进行了评估。DDE加速了雌性青春期的开始，并减少了雄性生殖行为。性腺形态和精子活力似乎未受影响。本研究结果表明，与成年后污染物暴露后传统的生殖成功指标相比，神经内分泌系统可能对胚胎期暴露于污染物更为敏感且恢复能力更弱。本研究进一步支持在毒性试验中纳入行为评估。
