Wu Zhongyi, Zhang Gaohua, Zhao Ran, Gao Qi, Zhao Jinchen, Zhu Xiaoxu, Wang Fangyan, Kang Zhensheng, Wang Xiaojing
State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, 712100, Shaanxi, China.
State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, 712100, Shaanxi, China.
Stress Biol. 2023 Oct 23;3(1):44. doi: 10.1007/s44154-023-00115-z.
Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is a catastrophic disease that threatens global wheat yield. Yr10 is a race-specific all-stage disease resistance gene in wheat. However, the resistance mechanism of Yr10 is poorly characterized. Therefore, to elucidate the potential molecular mechanism mediated by Yr10, transcriptomic sequencing was performed at 0, 18, and 48 h post-inoculation (hpi) of compatible wheat Avocet S (AvS) and incompatible near-isogenic line (NIL) AvS + Yr10 inoculated with Pst race CYR32. Respectively, 227, 208, and 4050 differentially expressed genes (DEGs) were identified at 0, 18, and 48 hpi between incompatible and compatible interaction. The response of Yr10 to stripe rust involved various processes and activities, as indicated by the results of Gene Ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. Specifically, the response included photosynthesis, defense response to fungus, metabolic processes related to salicylic acid (SA) and jasmonic acid (JA), and activities related to reactive oxygen species (ROS). Ten candidate genes were selected for qRT-PCR verification and the results showed that the transcriptomic data was reliable. Through the functional analysis of candidate genes by the virus-induced gene silencing (VIGS) system, it was found that the gene TaHPPD (4-hydroxyphenylpyruvate dioxygenase) negatively regulated the resistance of wheat to stripe rust by affecting SA signaling, pathogenesis-related (PR) gene expression, and ROS clearance. Our study provides insight into Yr10-mediated resistance in wheat.
条锈病由条形柄锈菌小麦专化型（Pst）引起，是一种威胁全球小麦产量的灾难性病害。Yr10是小麦中一个具有小种特异性的全生育期抗病基因。然而，Yr10的抗病机制尚不清楚。因此，为阐明Yr10介导的潜在分子机制，对接种条锈菌小种CYR32的感病小麦品种阿沃塞特S（AvS）和近等基因系阿沃塞特S+Yr10（AvS+Yr10）在接种后0、18和48小时（hpi）进行了转录组测序。在不相容和相容互作的0、18和48 hpi分别鉴定出227、208和4050个差异表达基因（DEG）。基因本体论（GO）富集分析和京都基因与基因组百科全书（KEGG）通路分析结果表明，Yr10对条锈病的反应涉及各种过程和活动。具体而言，该反应包括光合作用、对真菌的防御反应、与水杨酸（SA）和茉莉酸（JA）相关的代谢过程以及与活性氧（ROS）相关的活动。选择10个候选基因进行qRT-PCR验证，结果表明转录组数据可靠。通过病毒诱导基因沉默（VIGS）系统对候选基因进行功能分析，发现基因TaHPPD（4-羟基苯丙酮酸双加氧酶）通过影响SA信号传导、病程相关（PR）基因表达和ROS清除来负调控小麦对条锈病的抗性。我们的研究为小麦中Yr10介导的抗性提供了见解。
