Lee Mei-Hsien, Jiang Cheun-Bin, Juan Shu-Hui, Lin Rong-Dih, Hou Wen-Chi
Graduate Institute of Pharmacognosy, Taipei Medical University, Taiwan.
Fitoterapia. 2006 Feb;77(2):109-15. doi: 10.1016/j.fitote.2005.11.012. Epub 2006 Jan 3.
Recent studies have shown biological effects of heme oxygenase-1 (HO-1) induction and antioxidation in cardiovascular disorders. The ethanol extracts of leaves of 12 selected indigenous Taiwanese plants were investigated for their antioxidant activities, evaluated using assays of 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl, and superoxide radicals scavenging and reducing power activities as well as the induction of heme oxygenase-1 (HO-1). Acer albopurpurascens, Cinnamomum kanehirai, Diospyros discolor, Excoecaria kawakamii, Koelreuteria henryi, and Syzygium formosanum showed better DPPH-scavenging activities than the other plants. IC(50) values ranged from 1.7 to 8.7 microg/mL. Excepting Millettia pulchra var. microphylla and Pittosporum moluccanum, the extracts displayed hydroxyl-scavenging activities (IC(50) of 0.16-0.67 microg/mL). A. albopurpurascens, D. discolor, K. henryi, and S. formosanum also showed good superoxide anion radical scavenging activities and IC(50) values ranged from 12.9 to 28.5 microg/mL. D. discolor, K. henryi, and S. formosanum showed potent reducing power and M. pulchra var. microphylla and S. formosanum exhibited potent HO-1 induced activity. These active plant extracts also contained abundant phenolic constituents. The present results provide candidates to isolate the active constituents and develop natural antioxidants.
最近的研究表明，血红素加氧酶-1（HO-1）的诱导及其抗氧化作用在心血管疾病中具有生物学效应。对12种选定的台湾本土植物叶片的乙醇提取物进行了抗氧化活性研究，通过1,1-二苯基-2-苦基肼（DPPH）、羟基和超氧阴离子自由基清除及还原能力测定以及血红素加氧酶-1（HO-1）诱导试验进行评估。紫红叶槭、红楠、乌柿、川上氏海漆、湖北栾树和台湾蒲桃的DPPH清除活性优于其他植物。半数抑制浓度（IC50）值在1.7至8.7微克/毫升之间。除小叶红豆和摩鹿加海桐外，提取物均表现出羟基清除活性（IC50为0.16至0.67微克/毫升）。紫红叶槭、乌柿、湖北栾树和台湾蒲桃也表现出良好的超氧阴离子自由基清除活性，IC50值在12.9至28.5微克/毫升之间。乌柿、湖北栾树和台湾蒲桃表现出较强的还原能力，小叶红豆和台湾蒲桃表现出较强的HO-1诱导活性。这些活性植物提取物还含有丰富的酚类成分。目前的研究结果为分离活性成分和开发天然抗氧化剂提供了候选物。
