Chen Chin-Hui, Chan Hsiu-Chen, Chu Yi-Tsu, Ho Hsin-Yi, Chen Pi-Yu, Lee Tzong-Huei, Lee Ching-Kuo
College of Pharmacy, Taipei Medical University, 250 Wu Xin Street, Taipei 11031,Taiwan.
Molecules. 2009 Aug 10;14(8):2947-58. doi: 10.3390/molecules14082947.
Natural products have the potential to be developed into new drugs for the treatment of various diseases. The aim of the present study was to screen the antioxidant activities of some common edible fruits, garden plants and medicinal plants indigenous to Taiwan. This was performed by assessing the activities of lipoxygenase, xanthine oxidase and tyrosinase following incubation with extracts from these plants. A further aim was to use HPLC-DAD and tyrosinase to chromatographically identify the antioxidative constituents obtained from an extract exhibiting strong antioxidative properties. The acetone extracts of 27 cultivated plant species from Taiwan were tested for antioxidant activities towards xanthine oxidase, tyrosinase and lipoxygenase using spectrophotometric assays. Koelreuteria henryi, Prunus campanulata, and Rhodiola rosea showed the highest xanthine oxidase inhibitory activities. Camellia sinensis, Rhodiola rosea, and Koelreuteria henryi exhibited good tyrosinase inhibitory activities and potent anti-lipoxygenase activities. As Koelreuteria henryi had notable significant inhibitory activities towards xanthine oxidase, tyrosinase, and lipoxygenase, it was further tested with tyrosinase and HPLC-DAD. The results from this part of the study revealed that the more powerful the antioxidant capability of the extracted component, the greater the decrease in peak height obtained after reacting with tyrosinase. Additional studies are warranted to further characterize the compounds responsible for the antioxidant properties of the examined extracts.
天然产物有潜力被开发成治疗各种疾病的新药。本研究的目的是筛选台湾本土一些常见食用水果、园林植物和药用植物的抗氧化活性。这是通过评估这些植物提取物孵育后脂氧合酶、黄嘌呤氧化酶和酪氨酸酶的活性来进行的。另一个目的是使用高效液相色谱 - 二极管阵列检测器（HPLC - DAD）和酪氨酸酶对具有强抗氧化性能的提取物中获得的抗氧化成分进行色谱鉴定。使用分光光度法测定了来自台湾的27种栽培植物物种的丙酮提取物对黄嘌呤氧化酶、酪氨酸酶和脂氧合酶的抗氧化活性。台湾栾树、钟花樱桃和红景天表现出最高的黄嘌呤氧化酶抑制活性。茶树、红景天和台湾栾树表现出良好的酪氨酸酶抑制活性和强大的抗脂氧合酶活性。由于台湾栾树对黄嘌呤氧化酶、酪氨酸酶和脂氧合酶具有显著的抑制活性，因此进一步用酪氨酸酶和HPLC - DAD对其进行了测试。该研究这部分的结果表明，提取成分的抗氧化能力越强，与酪氨酸酶反应后获得的峰高降低幅度越大。有必要进行进一步的研究，以进一步表征所检测提取物中具有抗氧化特性的化合物。
