Bakir Mohammad Abdul, Kitahara Maki, Sakamoto Mitsuo, Matsumoto Mitsuharu, Benno Yoshimi
Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Center, Wako, Saitama 351-0198, Japan.
Int J Syst Evol Microbiol. 2006 Jan;56(Pt 1):151-4. doi: 10.1099/ijs.0.63914-0.
During studies of the microbiota of human faeces, five strains of Gram-negative anaerobic rods were isolated following growth in a polyamine-deficient medium. These strains belonged to the genus Bacteroides on the basis of 16S rRNA gene sequence data. 16S rRNA gene sequence similarity between one of the strains, 341T, and recognized species within the genus Bacteroides was <95 %. The DNA G+C content (44 mol%) and major fatty acid composition (anteiso-C(15 : 0), 32.0 %) supported the affiliation of strain 341(T) to the genus Bacteroides. Biochemical tests and DNA-DNA hybridization analysis demonstrated that strain 341T was distinct from Bacteroides uniformis and Bacteroides helcogenes, to which it was related most closely. On the basis of these data, a novel Bacteroides species, Bacteroides intestinalis sp. nov., is proposed with strain 341T (= JCM 13265T = DSM 17393T) as the type strain.
在对人类粪便微生物群的研究中，在缺乏多胺的培养基中培养后，分离出了5株革兰氏阴性厌氧杆菌。根据16S rRNA基因序列数据，这些菌株属于拟杆菌属。其中一株菌株341T与拟杆菌属内已确认的物种之间的16S rRNA基因序列相似性小于95%。DNA G+C含量（44 mol%）和主要脂肪酸组成（anteiso-C15:0，32.0%）支持菌株341T属于拟杆菌属。生化试验和DNA-DNA杂交分析表明，菌株341T与最密切相关的单形拟杆菌和生癌拟杆菌不同。基于这些数据，提出了一个新的拟杆菌物种——肠道拟杆菌，模式菌株为341T（=JCM 13265T=DSM 17393T）。
