Ge B, Girard W, Zhao S, Friedman S, Gaines S A, Meng J
Department of Nutrition and Food Science, University of Maryland, College Park, MD 20742, USA.
J Appl Microbiol. 2006;100(1):175-84. doi: 10.1111/j.1365-2672.2005.02750.x.
To determine the genetic relatedness of Campylobacter spp. from retail meat products, and compare the discriminatory power of pulsed-field gel electrophoresis (PFGE) and automatic ribotyping.
A total of 378 Campylobacter isolates recovered from 159 raw meats (130 chicken, 25 turkey, three pork and one beef) sampled from 50 retail grocery stores of four supermarket chains in the Maryland suburban area from August 1999 to July 2000 were analysed by PFGE with SmaI, 120 isolates of which were also characterized by ribotyping with PstI using RiboPrinter system. A total of 148 unique PFGE patterns were identified, 91 of which were present in multiple Campylobacter isolates and 24 in multiple meat samples. Nineteen Campylobacter clones with identical PFGE patterns recurred frequently (up to nine times) throughout the sampling period. Comparing ribotyping with PFGE, we identified 44 PFGE patterns and 22 RiboGroups among the 120 isolates tested. Multiple PFGE patterns within one RiboGroup were commonly observed, as well as multiple RiboGroups within one PFGE pattern.
Although Campylobacter present in retail meats were genetically diverse, certain clones persisted in poultry meats. PFGE had a greater discriminatory power than ribotyping, and the two methods were complementary in genotyping Campylobacter.
Genomic DNA fingerprinting of Campylobacter confirmed diverse and recurrent Campylobacter clones in the retail meats, which provides additional data for a better understanding of the epidemiological aspect of Campylobacter infection.
确定零售肉类产品中弯曲杆菌属的遗传相关性，并比较脉冲场凝胶电泳（PFGE）和自动核糖体分型的鉴别能力。
1999年8月至2000年7月期间，从马里兰州郊区四个超市连锁店的50家零售杂货店采集了159份生肉（130份鸡肉、25份火鸡肉、3份猪肉和1份牛肉），从中分离出378株弯曲杆菌，用SmaI进行PFGE分析，其中120株还使用RiboPrinter系统用PstI进行核糖体分型。共鉴定出148种独特的PFGE图谱，其中91种存在于多个弯曲杆菌分离株中，24种存在于多个肉类样本中。19个具有相同PFGE图谱的弯曲杆菌克隆在整个采样期间频繁出现（多达9次）。比较核糖体分型和PFGE，在测试的120株分离株中，我们鉴定出44种PFGE图谱和22个核糖体分型组。在一个核糖体分型组中通常观察到多种PFGE图谱，在一个PFGE图谱中也观察到多个核糖体分型组。
虽然零售肉类中的弯曲杆菌在遗传上具有多样性，但某些克隆在家禽肉中持续存在。PFGE的鉴别能力比核糖体分型更强，这两种方法在弯曲杆菌基因分型中具有互补性。
弯曲杆菌的基因组DNA指纹图谱证实了零售肉类中弯曲杆菌克隆的多样性和复发性，为更好地了解弯曲杆菌感染的流行病学方面提供了更多数据。
