He Wenfei, Liu Gang, Chen Xin, Lu Jincai, Abe Hidehiro, Huang Kexin, Manabe Masanobu, Kodama Hiroyuki
Department of Anesthesiology and Critical Care Medicine, Kochi Medical School, Nankoku-city, Kochi, Japan.
J Agric Food Chem. 2008 Mar 26;56(6):1921-7. doi: 10.1021/jf073364k. Epub 2008 Feb 26.
The effects of five ginsenosides (G-Rh2, -Rd, -Rb1, -Rb2, -Rh1) isolated from the root of Panax gingseng on stimulus-induced superoxide generation in human neutrophils were evaluated by measuring the reduction of ferricytochrome c. The tyrosyl or serine/threonine phosphorylation of neutrophil proteins and translocation of p47phox, p67phox, and Rac to the plasma membrane were detected using specific monoclonal antibodies. G-Rh2 significantly suppressed superoxide generation induced by N-formylmethionyl-leucylphenylalanine (fMLP), phorbol 12-myristate 13-acetate (PMA), and arachidonic acid (AA) in a concentration-dependent manner. G-Rh1 showed a comparably lower suppression on fMLP-induced superoxide generation. G-Rd, -Rb1, and -Rb2 also suppressed AA-induced superoxide generation in high concentrations. G-Rd and G-Rb1 showed no effect on fMLP- and PMA-induced superoxide generation. FMLP-, PMA-, and AA-induced tyrosyl or serine/threonine phosphorylation and translocation of p47phox, p67phox, and Rac to the plasma membrane were in parallel with the suppression of the stimulus-induced superoxide generation.
通过测量高铁细胞色素c的还原，评估了从人参根中分离出的五种人参皂苷（G-Rh2、-Rd、-Rb1、-Rb2、-Rh1）对人中性粒细胞刺激诱导的超氧化物生成的影响。使用特异性单克隆抗体检测中性粒细胞蛋白的酪氨酸或丝氨酸/苏氨酸磷酸化以及p47phox、p67phox和Rac向质膜的转位。G-Rh2以浓度依赖性方式显著抑制了由N-甲酰甲硫氨酰-亮氨酰-苯丙氨酸（fMLP）、佛波酯12-肉豆蔻酸酯13-乙酸酯（PMA）和花生四烯酸（AA）诱导的超氧化物生成。G-Rh1对fMLP诱导的超氧化物生成的抑制作用相对较低。G-Rd、-Rb1和-Rb2在高浓度时也抑制了AA诱导的超氧化物生成。G-Rd和G-Rb1对fMLP和PMA诱导的超氧化物生成没有影响。FMLP、PMA和AA诱导的酪氨酸或丝氨酸/苏氨酸磷酸化以及p47phox、p67phox和Rac向质膜的转位与刺激诱导的超氧化物生成的抑制作用平行。
