Zhang Mei, Liu Gang, Tang Shuhan, Song Shaojiang, Yamashita Koichi, Manabe Masanobu, Kodama Hiroyuki
Department of Anesthesiology and Critical Care Medicine, Kochi Medical School, Nankoku-shi, Kochi 783-8505, Japan.
Planta Med. 2006 Oct;72(13):1216-22. doi: 10.1055/s-2006-951679. Epub 2006 Oct 4.
The buds of Aralia elata (Miq.) Seem (Japanese angelica tree) have long been used as a tonic, antiarthritic and antidiabetic agent in China and Japan. We have isolated five triterpenoids, congmuyanosides A, C, D, echinocystic acid and 3-O-[beta-D-glucopyranosyl(1-->2)-beta-D-glucopyranosyl]-hederagenin from the buds of Aralia elata , and investigated their effects on stimulus-induced superoxide generation in human neutrophils. Congmuyanoside A, echinocystic acid and 3-O-[beta-D-glucopyranosyl(1-->2)-beta-D-glucopyranosyl]-hederagenin suppressed the superoxide generation induced by N-formyl-methionyl-leucyl-phenylalanine (fMLP) in a concentration-dependent manner. Congmuyanosides C, D and echinocystic acid significantly suppressed the superoxide generation induced by phorbol 12-myristate 13-acetate (PMA) and arachidonic acid (AA). The compounds also suppressed fMLP- and AA-induced tyrosyl or PMA-induced serine/threonine phosphorylation and translocation of cytosolic compounds, p47 (phox), p67 (phox) and Rac to the cell membrane.
辽东楤木（Aralia elata (Miq.) Seem，即刺老芽）的芽在中国和日本长期以来被用作滋补品、抗关节炎和抗糖尿病药物。我们从辽东楤木的芽中分离出了五种三萜类化合物，即葱木皂苷A、C、D、紫花前胡酸和3 - O - [β - D - 吡喃葡萄糖基(1→2)-β - D - 吡喃葡萄糖基]-常春藤皂苷元，并研究了它们对人中性粒细胞刺激诱导的超氧化物生成的影响。葱木皂苷A、紫花前胡酸和3 - O - [β - D - 吡喃葡萄糖基(1→2)-β - D - 吡喃葡萄糖基]-常春藤皂苷元以浓度依赖的方式抑制了N - 甲酰 - 甲硫氨酰 - 亮氨酰 - 苯丙氨酸（fMLP）诱导的超氧化物生成。葱木皂苷C、D和紫花前胡酸显著抑制了佛波醇12 - 肉豆蔻酸酯13 - 乙酸酯（PMA）和花生四烯酸（AA）诱导的超氧化物生成。这些化合物还抑制了fMLP和AA诱导的酪氨酸磷酸化或PMA诱导的丝氨酸/苏氨酸磷酸化以及胞质化合物p47（phox）、p67（phox）和Rac向细胞膜的转位。
