Chen Xin, Lu Jincai, He Wenfei, Chi Haidong, Yamashita Koichi, Manabe Masanobu, Kodama Hiroyuki
Department of Medicinal Plants, Shenyang Pharmaceutical University, Shenyang, 110016, China.
Fitoterapia. 2009 Mar;80(2):105-11. doi: 10.1016/j.fitote.2008.11.003. Epub 2008 Nov 19.
Four triterpenoid compounds hederacolchiside E (1), hederasaponin B (2), raddeanoside 20 (3) and raddeanoside 21 (4) were isolated from ethanol extracts of rhizome of Anemone raddeana Regel. The effects of these triterpenoids on superoxide generation, tyrosyl phosphorylation of proteins and translocation of cytosolic compounds, such as p47(phox), p67(phox) and Rac to the cell membrane in human neutrophils was investigated. The superoxide generation induced by N-formyl-methionyl-leucyl-phenylalanine (fMLP) was slightly suppressed by hederasaponin B, raddeanoside 20 and raddeanoside 21 in a concentration dependent manner. The superoxide generation induced by arachidonic acid (AA) was suppressed by hederasaponin B and raddeanoside 21 significantly. fMLP- and AA-induced tyrosyl phosphorylation and translocation of the cytosolic proteins: p47(phox), p67(phox), and Rac to the cell membrane were suppressed in parallel with the suppression of stimulus-induced superoxide generation.
从毛茛科植物林荫银莲花（Anemone raddeana Regel）根茎的乙醇提取物中分离得到了四种三萜类化合物：常春藤皂苷元E（1）、常春藤皂苷B（2）、红景天苷20（3）和红景天苷21（4）。研究了这些三萜类化合物对人中性粒细胞中超氧化物生成、蛋白质酪氨酸磷酸化以及胞质化合物（如p47(phox)、p67(phox)和Rac）向细胞膜转位的影响。常春藤皂苷B、红景天苷20和红景天苷21以浓度依赖的方式对N-甲酰甲硫氨酰-亮氨酰-苯丙氨酸（fMLP）诱导的超氧化物生成有轻微抑制作用。常春藤皂苷B和红景天苷21对花生四烯酸（AA）诱导的超氧化物生成有显著抑制作用。fMLP和AA诱导的酪氨酸磷酸化以及胞质蛋白p47(phox)、p67(phox)和Rac向细胞膜的转位与刺激诱导的超氧化物生成的抑制同时受到抑制。
