Funatsu Hideharu, Yamashita Hidetoshi, Nakamura Shinko, Mimura Tatsuya, Eguchi Shuichiro, Noma Hidetaka, Hori Sadao
Department of Ophthalmology, Diabetes Center, Tokyo Women's Medical University, Tokyo, Japan.
Ophthalmology. 2006 Feb;113(2):294-301. doi: 10.1016/j.ophtha.2005.10.030. Epub 2006 Jan 10.
To investigate whether vitreous levels of vascular endothelial growth factor (VEGF) and pigment epithelium-derived factor (PEDF) are related to diabetic macular edema (DME).
Retrospective case-control study.
Thirty-six patients with DME, 6 diabetic patients without retinopathy, and 13 patients with nondiabetic ocular disease.
After vitreous fluid samples were obtained at vitreoretinal surgery, VEGF and PEDF levels in the vitreous fluid were measured by enzyme-linked immunosorbent assay.
Correlations between vascular permeability and the vitreous fluid levels of VEGF and PEDF.
The vitreous level of VEGF was significantly higher in patients with DME than in nondiabetic patients and diabetic patients without retinopathy (P<0.0001 and P<0.0001, respectively). Conversely, the vitreous level of PEDF was significantly lower in patients with DME than in nondiabetic patients and diabetic patients without retinopathy (P<0.0001 and P<0.0001, respectively). The vitreous level of PEDF did not correlate significantly with that of VEGF (P = 0.1806). The vitreous level of VEGF was significantly higher in patients with hyperfluorescent DME than in those with minimally fluorescent DME (P = 0.0022). Conversely, the vitreous PEDF level was significantly lower in patients with hyperfluorescent DME than in those with minimally fluorescent DME (P = 0.0172). Vitreous levels of VEGF and PEDF were related to the retinal thickness at the central fovea (P<0.0001 and P = 0.0469, respectively).
Our retrospective study suggests that VEGF and PEDF have an independent association with vascular permeability in the eye and on the DME, and we recommend that prospective validation of our findings be undertaken to confirm these observations.
研究玻璃体内血管内皮生长因子（VEGF）和色素上皮衍生因子（PEDF）水平是否与糖尿病性黄斑水肿（DME）相关。
回顾性病例对照研究。
36例DME患者、6例无视网膜病变的糖尿病患者和13例非糖尿病性眼病患者。
在玻璃体视网膜手术中获取玻璃体液样本后，采用酶联免疫吸附测定法测量玻璃体液中VEGF和PEDF水平。
血管通透性与玻璃体液中VEGF和PEDF水平之间的相关性。
DME患者玻璃体内VEGF水平显著高于非糖尿病患者和无视网膜病变的糖尿病患者（分别为P<0.0001和P<0.0001）。相反，DME患者玻璃体内PEDF水平显著低于非糖尿病患者和无视网膜病变的糖尿病患者（分别为P<0.0001和P<0.0001）。玻璃体内PEDF水平与VEGF水平无显著相关性（P = 0.1806）。荧光素高渗漏性DME患者玻璃体内VEGF水平显著高于荧光素低渗漏性DME患者（P = 0.0022）。相反，荧光素高渗漏性DME患者玻璃体内PEDF水平显著低于荧光素低渗漏性DME患者（P = 0.0172）。玻璃体内VEGF和PEDF水平与中央凹处视网膜厚度相关（分别为P<0.0001和P = 0.0469）。
我们的回顾性研究表明，VEGF和PEDF与眼部血管通透性及DME存在独立关联，我们建议对我们的研究结果进行前瞻性验证以证实这些观察结果。
