Kostoulas P, Leontides L, Billinis C, Amiridis G S, Florou M
Laboratory of Epidemiology, Biostatistics and Animal Health Economics, School of Veterinary Medicine, University of Thessaly, 224 Trikalon st., 43100 Karditsa, Greece.
Prev Vet Med. 2006 May 17;74(2-3):226-38. doi: 10.1016/j.prevetmed.2005.12.001. Epub 2006 Jan 9.
Our cross-sectional study investigated the association of sub-clinical Mycobacterium avium subsp. paratuberculosis (MAP) infection with failing to produce a live offspring the season of lambing/kidding (November 2001 to January 2002) before testing (in April-May 2002), in four dairy-sheep and/or goat flocks in Greece (369 animals >or=1.5-year-old). From each selected animal 10 ml of blood and 10 g of feces from the rectum were obtained. The harvested sera were tested for antibodies to MAP with a commercial ELISA test kit; the feces were cultured on Herrold's egg-yolk medium supplemented with mycobactin J and antibiotics. An animal was considered sub-clinically infected when found either seropositive or culture positive. The true prevalence of sub-clinically infected animals, adjusted for the sensitivity and specificity of the parallel testing, was 14% (0.1-28%) and 35.9% (9.2-62.7%) in sheep and goats, respectively. The association of fertility of sheep and goats with sub-clinical paratuberculosis was investigated in random-effects logistic models. Sub-clinically infected animals (compared to uninfected) had OR for live offspring the previous year of 5.4 for parity <4, OR=0.05 for parity >6, and a non-significant OR for the middle parity category.
我们的横断面研究调查了希腊四个奶羊和/或山羊群（369只年龄≥1.5岁的动物）中，亚临床副结核分枝杆菌（MAP）感染与在产羔/产仔季节（2001年11月至2002年1月）且在检测前（2002年4月至5月）未产下活仔之间的关联。从每只选定的动物身上采集10毫升血液和10克直肠粪便。采集的血清用商用ELISA检测试剂盒检测MAP抗体；粪便在添加了分枝杆菌素J和抗生素的赫罗尔德蛋黄培养基上培养。当动物血清呈阳性或培养呈阳性时，被认为是亚临床感染。经平行检测的敏感性和特异性校正后，绵羊和山羊中亚临床感染动物的实际患病率分别为14%（0.1 - 28%）和35.9%（9.2 - 62.7%）。在随机效应逻辑模型中研究了绵羊和山羊的繁殖力与亚临床副结核病的关联。亚临床感染动物（与未感染动物相比）在前一年产活仔的比值比，初产次数<4时为5.4，初产次数>6时为0.05，中间初产次数类别无显著比值比。
