Rawther Sulficar Shamsudeen, Saseendranath Mapranath Raghavan, Nair Govinda Pilla Krishnan, Tresamol Palathingal Vareid, Pillai Usha Narayana, Abraham John, Senthilkumar Tuticorin Maragatham Alagesan, Nagalakshmy Shrine, Nimisha Krishna Kripa Narayanan
Department of Veterinary Epidemiology and Preventive Medicine, College of Veterinary and Animal Sciences, Mannuthy, Trichur, Kerala, India.
Trop Anim Health Prod. 2012 Apr;44(4):911-4. doi: 10.1007/s11250-011-9986-y. Epub 2011 Sep 29.
In the present study efficacy of single intradermal Johnin test, acid fast staining of faecal smear and IS 900 faecal polymerase chain reaction tests was evaluated in 200 goats for detection of Mycobacterium avium subsp paratuberculosis. Two hundred goats comprising 150 goats from an organised farm in Trichur district and 50 goats reared under field condition at farmers premise from Malappuram district of Kerala state formed the study population. Faecal smear from all the 200 goats was stained by Ziehl-Neelsen acid fast stain and faecal polymerase chain reaction (PCR) specific for M. avium subsp paratuberculosis (MAP); IS 900 was performed on all samples. All the animals were subjected to single intradermal Johnin test. Out of 200 goats screened for paratuberculosis, six goats (3%), 11 goats (5.5%) and 42 goats (21%) were found positive by Ziehl-Neelsen acid fast staining of faecal smear, single intradermal Johnin test and IS 900 PCR respectively. Results of the present study indicate that amplification of IS 900 insertion element was the most specific and sensitive diagnostic detection method. Single intradermal Johnin test and Ziehl-Neelsen acid fast staining did not show any significant difference.
在本研究中，对200只山羊进行了皮内注射琼宁试验、粪便涂片抗酸染色和IS 900粪便聚合酶链反应检测，以检测副结核分枝杆菌。200只山羊组成了研究群体，其中150只来自特里丘尔区一个有组织的农场，50只在喀拉拉邦马拉普拉姆区农民家中的田间条件下饲养。对所有200只山羊的粪便涂片进行齐-尼抗酸染色，并对副结核分枝杆菌（MAP）进行粪便聚合酶链反应（PCR）；对所有样本进行IS 900检测。所有动物均接受皮内注射琼宁试验。在筛查副结核的200只山羊中，粪便涂片齐-尼抗酸染色、皮内注射琼宁试验和IS 900 PCR分别检测出6只山羊（3%）、11只山羊（5.5%）和42只山羊（21%）呈阳性。本研究结果表明，IS 900插入元件的扩增是最特异、最敏感的诊断检测方法。皮内注射琼宁试验和齐-尼抗酸染色未显示出任何显著差异。
