Meyer Hellen, Bolarinwa Adrian, Wolfram Guenther, Linseisen Jakob
Unit of Human Nutrition and Cancer Prevention, Technical University of Munich, Freising-Weihenstephan, Germany.
Ann Nutr Metab. 2006;50(3):167-72. doi: 10.1159/000090736. Epub 2006 Jan 10.
Absorption and excretion of apigenin after the ingestion of apiin-rich food, i.e. parsley, was tested.
Eleven healthy subjects (5 women, 6 men) in the age range of 23-41 years and with an average body mass index of 23.9 +/- 4.1 kg/m2 took part in this study. After an apigenin- and luteolin-free diet, a single oral bolus of 2 g blanched parsley (corresponding to 65.8 +/- 15.5 micromol apigenin) per kilogram body weight was consumed. Blood samples were taken at 0, 4, 6, 7, 8, 9, 10, 11 and 28 h after parsley consumption and 24-hour urine samples were collected. Apigenin was analyzed in plasma, urine and red blood cells by means of HPLC-ECD.
On average, a maximum apigenin plasma concentration of 127 +/- 81 nmol/l was reached after 7.2 +/- 1.3 h with a high range of variation between subjects. For all participants, plasma apigenin concentration rose after bolus ingestion and fell within 28 h under the detection limit (2.3 nmol/l). The average apigenin content in 24-hour urine was 144 +/- 110 nmol/24 h corresponding to 0.22 +/- 0.16% of the ingested dose. The flavone could be detected in red blood cells without showing dose-response characteristics.
A small portion of apigenin provided by food reaches the human circulation and, therefore, may reveal biological effects.
测试摄入富含芹菜苷的食物（即欧芹）后芹菜素的吸收和排泄情况。
11名年龄在23 - 41岁之间、平均体重指数为23.9±4.1kg/m²的健康受试者（5名女性，6名男性）参与了本研究。在食用不含芹菜素和木犀草素的饮食后，每千克体重单次口服2g焯过的欧芹（相当于65.8±15.5微摩尔芹菜素）。食用欧芹后0、4、6、7、8、9、10、11和28小时采集血样，并收集24小时尿液样本。通过高效液相色谱 - 电化学检测法分析血浆、尿液和红细胞中的芹菜素。
平均而言，7.2±1.3小时后芹菜素血浆浓度达到最高，为127±81nmol/l，受试者之间差异范围较大。对于所有参与者，单次摄入后血浆芹菜素浓度升高，并在28小时内降至检测限（2.3nmol/l）以下。24小时尿液中芹菜素的平均含量为144±110nmol/24小时，相当于摄入剂量的0.22±0.16%。在红细胞中可检测到黄酮类化合物，但未显示出剂量反应特征。
食物提供的一小部分芹菜素进入人体循环，因此可能具有生物学效应。
