蝙蝠葛酚性碱对血栓形成及血小板聚集的影响
[Effect of phenolic alkaloids of Menispermum dauricum on thrombosis and platelet aggregation].
作者信息
Kong Xiang-Ying, Gong Pei-Li
机构信息
Department of Pharmacology, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, China.
出版信息
Yao Xue Xue Bao. 2005 Oct;40(10):916-9.
AIM
To observe the effect of phenolic alkaloids of Menispermum dauricum (PAMD) on thrombosis and platelet aggregation, and to explore its mechanism of action.
METHODS
Thrombosis was observed with arteriovenous shunt thrombus model in rat; platelet aggregation was determined by Born's method; ultrastructure of platelet was observed by transmission electron microscope; TXB2 or 6-keto-PGF1alpha levels were assessed by radioimmunoassay; and NO was determined by colorimetric method.
RESULTS
PAMD dose-dependently inhibited experimental thrombus formation, platelet aggregation induced by ADP, AA and THR in vivo and ultrastructure changes stimulated by THR; PAMD increased the generation of 6-keto-PGF1alpha in thoracic aortae and NO level in plasma; and had no influence on TXB2 release (P > 0.05).
CONCLUSION
PAMD inhibited thrombosis and platelet aggregation, and its mechanism might be due to the increase of PGI2 and NO level.
目的
观察蝙蝠葛酚性碱（PAMD）对血栓形成及血小板聚集的影响，并探讨其作用机制。
方法
采用大鼠动静脉旁路血栓模型观察血栓形成情况；用Born法测定血小板聚集；通过透射电子显微镜观察血小板超微结构；采用放射免疫分析法检测TXB2或6-酮-前列腺素F1α水平；用比色法测定一氧化氮（NO）。
结果
PAMD剂量依赖性地抑制实验性血栓形成、体内由二磷酸腺苷（ADP）、花生四烯酸（AA）和凝血酶（THR）诱导的血小板聚集以及由THR刺激引起的超微结构变化；PAMD增加胸主动脉中6-酮-前列腺素F1α的生成及血浆中NO水平；对TXB2释放无影响（P>0.05）。
结论
PAMD抑制血栓形成和血小板聚集，其机制可能是由于前列环素（PGI2）和NO水平升高。
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