Huang H L, Rao M R
Department of Cardiovascular Pharmacology, Nanjing Medical University.
Yao Xue Xue Bao. 1995;30(7):486-90.
The effects of neferine (Nef), taurine (Tau) and Nef + Tau on platelet aggregation and generation of thrombosis were investigated in rats. Using turbidimetry Nef and Tau were found to inhibit platelet aggregation induced by adenosine diphosphate (ADP), collagen or thrombin. Both drugs reduced the wet weight of experimental thrombosis. But the drugs showed no obvious effect on fibrinogen content and euglobulin lysis time. Thromboxane A2 (TXA2) production induced by ADP and prostacyclin (PGI2) were studied by enzyme immunoassay in rats. TXA2 generation in platelet rich plasma from rats treated with Nef and Nef + Tau obviously decreased. While the plasma PGI2 was not affected. Nef + Tau iv in 30 min significantly decreased the level of total cholesterin in serum. The results suggest that Nef has inhibitory effect on platelet aggregation and thrombosis formation. The combination of both drugs was found to be more potent than either one alone. The mechanism may be related to its reduction of TXA2 formation.
研究了甲基莲心碱（Nef）、牛磺酸（Tau）及Nef+Tau对大鼠血小板聚集和血栓形成的影响。采用比浊法发现，Nef和Tau可抑制二磷酸腺苷（ADP）、胶原或凝血酶诱导的血小板聚集。两种药物均降低了实验性血栓的湿重。但这两种药物对纤维蛋白原含量和优球蛋白溶解时间无明显影响。采用酶免疫分析法研究了大鼠中ADP诱导的血栓素A2（TXA2）生成及前列环素（PGI2）。用Nef和Nef+Tau处理的大鼠富含血小板血浆中的TXA2生成明显减少。而血浆PGI2未受影响。静脉注射Nef+Tau 30分钟后可显著降低血清总胆固醇水平。结果表明，Nef对血小板聚集和血栓形成具有抑制作用。发现两种药物联合使用比单独使用任何一种药物更有效。其机制可能与其减少TXA2形成有关。
