Ntziachristos Vasilis, Turner Gordon, Dunham Joshua, Windsor Stephen, Soubret Antoine, Ripoll Jorge, Shih Helen A
Massachusetts General Hospital & Harvard Medical School, Center for Molecular Imaging Research, Laboratory for Biooptics and Molecular Imaging, Charlestown, Massachusetts 02129, USA.
J Biomed Opt. 2005 Nov-Dec;10(6):064007. doi: 10.1117/1.2136148.
Fluorescence imaging of tissues has gained significant attention in recent years due to the emergence of appropriate reporter technologies that enable noninvasive sensing of molecular function in vivo. Two major approaches have been used so far for fluorescence molecular imaging, i.e., epi-illumination (reflectance) imaging and fluorescence molecular tomography. Transillumination is an alternative approach that has been employed for imaging tissues in the past and could be similarly beneficial for fluorescence molecular imaging. We investigate data normalization schemes in reflectance and transillumination mode and experimentally demonstrate that normalized transillumination offers significant advantages over planar reflectance imaging and over nonnormalized methods. Our observations, based on phantoms and on postmortem and in vivo mouse measurements display image quality improvement, superior depth sensitivity, and improved imaging accuracy over the nonnormalized methods examined. Normalized planar imaging retains implementation simplicity and could be used to improve on standard fluorescence reflectance imaging and as a simplified alternative to the more integrated and accurate tomographic methods.
近年来，由于出现了合适的报告技术，使得在体内能够对分子功能进行无创传感，组织荧光成像受到了广泛关注。到目前为止，荧光分子成像主要采用两种方法，即落射照明（反射）成像和荧光分子断层扫描。透照成像过去曾用于组织成像，是一种替代方法，同样可能对荧光分子成像有益。我们研究了反射和透照模式下的数据归一化方案，并通过实验证明，归一化透照比平面反射成像和非归一化方法具有显著优势。我们基于体模、死后和活体小鼠测量的观察结果显示，与所研究的非归一化方法相比，图像质量得到改善，深度灵敏度更高，成像精度也有所提高。归一化平面成像保持了实施的简单性，可用于改进标准荧光反射成像，并作为更综合、准确的断层扫描方法的简化替代方法。
