Kepshire Dax, Davis Scott C, Dehghani Hamid, Paulsen Keith D, Pogue Brian W
Thayer School of Engineering, Dartmouth College, Hanover, NH 03755, USA.
Opt Express. 2008 Jun 9;16(12):8581-93. doi: 10.1364/oe.16.008581.
Optical imaging of fluorescent objects embedded in a tissue simulating medium was characterized using non-contact based approaches to fluorescence remittance imaging (FRI) and sub-surface fluorescence diffuse optical tomography (FDOT). Using Protoporphyrin IX as a fluorescent agent, experiments were performed on tissue phantoms comprised of typical in-vivo tumor to normal tissue contrast ratios, ranging from 3.5:1 up to 10:1. It was found that tomographic imaging was able to recover interior inclusions with high contrast relative to the background; however, simple planar fluorescence imaging provided a superior contrast to noise ratio. Overall, FRI performed optimally when the object was located on or close to the surface and, perhaps most importantly, FDOT was able to recover specific depth information about the location of embedded regions. The results indicate that an optimal system for localizing embedded fluorescent regions should combine fluorescence reflectance imaging for high sensitivity and sub-surface tomography for depth detection, thereby allowing more accurate localization in all three directions within the tissue.
利用基于非接触的荧光反射成像（FRI）和表面下荧光扩散光学断层扫描（FDOT）方法，对嵌入组织模拟介质中的荧光物体进行光学成像表征。以原卟啉IX作为荧光剂，在由典型的体内肿瘤与正常组织对比度（范围从3.5:1到10:1）组成的组织模型上进行实验。结果发现，断层成像能够以相对于背景的高对比度恢复内部内含物；然而，简单的平面荧光成像提供了更高的对比度噪声比。总体而言，当物体位于表面上或靠近表面时，FRI表现最佳，也许最重要的是，FDOT能够恢复有关嵌入区域位置的特定深度信息。结果表明，用于定位嵌入荧光区域的最佳系统应结合用于高灵敏度的荧光反射成像和用于深度检测的表面下断层扫描，从而在组织内的所有三个方向上实现更准确的定位。
