[草乌提取物中乌头碱和新乌头碱的经皮吸收]
[Percutaneous absorption of aconitine and mesaconitine in extracts of Radix Aconitum kusnezoffii].
作者信息
Liu Zhi-Min, Niu Xin, Yang Xue-Zhi, Niu Shu-Dong
机构信息
Department of Functional Experiment, School of Preclinical Medicine, Beijing University of Traditional Chinese Medicine, Beijing 100029, China.
出版信息
Zhong Xi Yi Jie He Xue Bao. 2006 Jan;4(1):68-72. doi: 10.3736/jcim20060118.
OBJECTIVE
This study was conducted to evaluate the percutaneous absorption of aconitine and mesaconitine in extracts of Radix Aconitum kusnezoffii.
METHODS
The extracts of Radix Aconitum kusnezoffii were collected by using Franz diffusion cells after permeation through the skin of rats. Then rate constants of skin permeation of aconitine and mesaconitine were determined by high-performance liquid chromatography (HPLC).
RESULTS
Under the condition that the concentrations of azone and propylene glycol were both 4%, the cumulative doses of skin permeation (Q) of mesaconitine and aconitine in the extracts of Radix Aconitum kusnezoffii (600 mg/ml) for 24 hours were 165.819 and 487.747 microg/cm(2) respectively, and their rate constants of skin permeation were 18.391 and 78.805 microg.cm(-2).h(-1) respectively.
CONCLUSION
The aconitine and mesaconitine in the extracts of Radix Aconitum kusnezoffii can penetrate well through the skin of rats. Propylene glycol and azone can promote this penetration effects. The formula of skin permeation of mesaconitine and aconitine is in accordance with Higuchi equation and there is a linear relationship between Q and t(1/2).
目的
本研究旨在评价草乌提取物中乌头碱和中乌头碱的经皮吸收情况。
方法
采用Franz扩散池，以大鼠皮肤为渗透屏障，收集草乌提取物。然后用高效液相色谱法（HPLC）测定乌头碱和中乌头碱的皮肤渗透速率常数。
结果
在氮酮和丙二醇浓度均为4%的条件下，草乌提取物（600mg/ml）中中乌头碱和乌头碱24小时的皮肤累积渗透量（Q）分别为165.819和487.747μg/cm²，其皮肤渗透速率常数分别为18.391和78.805μg·cm⁻²·h⁻¹。
结论
草乌提取物中的乌头碱和中乌头碱能较好地透过大鼠皮肤。丙二醇和氮酮可促进这种渗透作用。中乌头碱和乌头碱的皮肤渗透符合Higuchi方程，且Q与t₁/₂呈线性关系。
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