Ma Ying, Qian Lei, Huang Haizhen, Yang Xiurong
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
J Colloid Interface Sci. 2006 Mar 15;295(2):583-8. doi: 10.1016/j.jcis.2005.05.031. Epub 2006 Jan 18.
A novel method for the fabrication of gold nanoparticle multilayer films based on the covalent-bonding interaction between boronic acid and polyols, poly(vinyl alcohol) (PVA), was developed. The multilayer buildup was monitored by UV-vis absorbance spectroscopy, which showed a linear increase of the film absorbance with the number of adsorbed Au layers and indicated the stepwise and uniform assembling process. The atomic force microscopy (AFM) image showed that a compact gold multilayer thin film was successfully assembled. The residual boronic acid group on the surface of thin film could incorporate glycosylated-protein horseradish peroxidase (HRP), and good catalytic activity for H2O2 could be observed.
基于硼酸与多元醇、聚乙烯醇（PVA）之间的共价键相互作用，开发了一种制备金纳米颗粒多层膜的新方法。通过紫外可见吸收光谱监测多层膜的形成过程，结果表明膜的吸光度随吸附金层的数量呈线性增加，表明组装过程是逐步且均匀的。原子力显微镜（AFM）图像显示成功组装了致密的金多层薄膜。薄膜表面残留的硼酸基团可以结合糖基化蛋白辣根过氧化物酶（HRP），并且可以观察到对H2O2具有良好的催化活性。
