Adamowicz Tatiana, Flisikowski Krzysztof, Starzyński Rafał, Zwierzchowski Lech, Switoński Marek
Department of Genetics and Animal Breeding, Agricultural University of Poznan, Poznan, Poland.
Mamm Genome. 2006 Jan;17(1):77-82. doi: 10.1007/s00335-005-0068-1. Epub 2006 Jan 13.
Leptin is expressed mainly by adipocytes and plays a crucial role in the regulation of energy expenditure, food intake, and adiposity. Using PCR-heteroduplex analysis and sequencing, we investigated a C/G substitution in the promoter region of the bovine leptin gene. Application of the electrophoretic mobility shift assay showed that the C-->G transversion decreased the leptin gene promoter binding capacity for nuclear proteins. With real-time PCR and Western blotting, we showed that the leptin expression level was higher in cattle with the CC than with the GG genotype.
瘦素主要由脂肪细胞表达，在能量消耗、食物摄入和肥胖的调节中起关键作用。我们利用聚合酶链反应-异源双链分析和测序技术，研究了牛瘦素基因启动子区域的一个C/G替换。电泳迁移率变动分析表明，C到G的颠换降低了瘦素基因启动子与核蛋白的结合能力。通过实时聚合酶链反应和蛋白质免疫印迹法，我们发现CC基因型牛的瘦素表达水平高于GG基因型牛。
