Moscatello David K, Dougherty Megan, Narins Rhoda S, Lawrence Naomi
Differentiated Cell Laboratory, Coriell Institute for Medical Research, 403 Haddon Avenue, Camden, NJ 08103, USA.
Dermatol Surg. 2005 Nov;31(11 Pt 2):1506-10. doi: 10.2310/6350.2005.31235.
Autologous fat transfer for soft tissue augmentation has been increasing in recent years. Graft longevity may vary greatly from patient to patient, requiring repeat procedures, often using frozen adipose tissue. Storage usually involves placing syringes of fat directly into a -20 degrees C freezer. However, the viability of fat frozen in this way is controversial.
This study tested methods for the optimal storage of adipose tissue harvested by tumescent liposuction.
Aliquots of washed adipose tissue were frozen directly at -20 degrees C or mixed with cryoprotectants, frozen at 1 degree C/min, and subsequently stored in liquid nitrogen vapor phase. Aliquots were subsequently thawed, and adipocyte viability was determined by staining and culture methods.
Viability of adipocytes frozen at -20 degrees C was very low when analyzed by staining, and no cultures could be established from any of the specimens. In contrast, viable adipocytes were recovered from samples that were controlled-rate frozen in the presence of cryoprotectants and stored in nitrogen vapor. CONCLUSION. Our results indicate that fat frozen at -20 degrees C is not viable and thus provides no advantage over inert fillers. The methods here described could readily be transferred to the clinical setting after further laboratory study.
近年来，自体脂肪移植用于软组织填充的情况日益增多。移植脂肪的留存时间在患者之间可能差异很大，这就需要重复进行手术，且常常使用冷冻的脂肪组织。储存通常是将装有脂肪的注射器直接放入-20℃的冰箱中。然而，以这种方式冷冻的脂肪的存活率存在争议。
本研究测试了肿胀吸脂获取的脂肪组织的最佳储存方法。
将洗涤后的脂肪组织等分试样直接在-20℃冷冻，或与冷冻保护剂混合，以1℃/分钟的速度冷冻，随后储存在液氮气相中。随后将等分试样解冻，并通过染色和培养方法测定脂肪细胞的存活率。
通过染色分析，在-20℃冷冻的脂肪细胞存活率非常低，且任何标本均无法建立培养物。相比之下，在冷冻保护剂存在下进行控速冷冻并储存在氮气气相中的样本中可回收存活的脂肪细胞。结论。我们的结果表明，在-20℃冷冻的脂肪没有活力，因此与惰性填充物相比没有优势。经过进一步的实验室研究后，这里描述的方法可以很容易地应用于临床。
