Hassan Raffit, Laszik Zoltan G, Lerner Megan, Raffeld Mark, Postier Russell, Brackett Daniel
Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892-4264, USA.
Am J Clin Pathol. 2005 Dec;124(6):838-45.
Mesothelin, a cell surface glycoprotein present on normal mesothelial cells, has been reported to be expressed in pancreatic adenocarcinomas. We conducted this study to fully characterize mesothelin expression in surgically resected, formalin-fixed, paraffin-embedded tissue specimens of 18 pancreatic adenocarcinomas, 9 adenocarcinomas of the ampulla of Vater, 12 adenocarcinomas of the common bile duct, and 17 cases of chronic pancreatitis. Mesothelin immunostaining was performed using the antimesothelin monoclonal antibody 5B2. All 18 cases (100%) of pancreatic adenocarcinomas showed mesothelin expression, as did 8 (89%) of 9 cases of ampullar adenocarcinoma and all 12 cases (100%) of common bile duct adenocarcinoma. In all cases of pancreaticobiliary adenocarcinoma, the adjacent normal pancreas did not stain for mesothelin. Of 17 specimens of chronic pancreatitis, 16 were negative for mesothelin expression, and 1 case showed weak mesothelin staining of fewer than 5% of normal pancreatic ducts. Our results demonstrated mesothelin expression in the majority of pancreaticobiliary adenocarcinomas and no expression in normal pancreatic tissues and in chronic pancreatitis.
间皮素是一种存在于正常间皮细胞表面的糖蛋白，据报道在胰腺腺癌中表达。我们开展这项研究，以全面表征间皮素在18例胰腺腺癌、9例 Vater 壶腹腺癌、12例胆总管腺癌以及17例慢性胰腺炎的手术切除、福尔马林固定、石蜡包埋组织标本中的表达情况。使用抗间皮素单克隆抗体5B2进行间皮素免疫染色。18例胰腺腺癌全部（100%）显示间皮素表达，9例壶腹腺癌中有8例（89%）显示间皮素表达，12例胆总管腺癌全部（100%）显示间皮素表达。在所有胰胆管腺癌病例中，相邻的正常胰腺组织间皮素染色阴性。17例慢性胰腺炎标本中，16例间皮素表达阴性，1例显示正常胰管中少于5%的细胞间皮素染色弱阳性。我们的结果表明，大多数胰胆管腺癌中间皮素表达，而正常胰腺组织和慢性胰腺炎中间皮素不表达。
