Zaldivar F, McMurray R G, Nemet D, Galassetti P, Mills P J, Cooper D M
Pediatric Exercise Research Center, University of California, Irvine, CA, USA.
Int J Obes (Lond). 2006 Jun;30(6):906-11. doi: 10.1038/sj.ijo.0803227.
To determine the effects of obesity on baseline levels of circulating granulocytes, monocytes, and lymphocyte subtypes in otherwise healthy children.
Two group comparison of leukocytes in normal weight control and overweight children.
In total, 38 boys and girls, ages 6-18 years, divided in two groups: normal weight, (NW, BMI<85th %tile, n=15) and overweight (OW, body mass index (BMI)>85th %tile, n=23).
BMI obtained from direct measures of height and body mass. Body fat was assessed by DEXA. Complete blood counts (CBC) were obtained by standard clinical hematology methods and surface antigen staining by flow cytometry.
The OW group compared to the NW group had increased total leukocytes counts (P=0.011), neutrophils (P=0.006), monocytes (P=0.008), total T (CD3) lymphocytes (P=0.022), and Helper T (CD4(+)) cells (P=0.003). Significant correlations were evident between leukocytes, and BMI percentile, BMI, or percent body fat. Neither lean body mass nor VO(2peak) per unit lean body mass were significantly related to any of the leukocytes. Percent body fat and BMI percentile were positively correlated (P<0.05) to total T cells (CD3) and/or helper T cells (CD4(+)).
A group of 23 overweight children displayed elevated counts in most types of circulating immune cells, suggesting the presence of low-grade systemic inflammation, a known pathogenetic mechanism underlying most long-term complications of obesity. Our data provide an additional rationale for the importance of avoiding or correcting pediatric obesity.
确定肥胖对其他方面健康的儿童循环粒细胞、单核细胞和淋巴细胞亚群基线水平的影响。
对正常体重对照组和超重儿童的白细胞进行两组比较。
总共38名6至18岁的男孩和女孩，分为两组：正常体重组（NW，BMI<第85百分位数，n = 15）和超重组（OW，体重指数（BMI）>第85百分位数，n = 23）。
通过直接测量身高和体重获得BMI。通过双能X线吸收法评估体脂。通过标准临床血液学方法获得全血细胞计数（CBC），并通过流式细胞术进行表面抗原染色。
与NW组相比，OW组的总白细胞计数（P = 0.011）、中性粒细胞（P = 0.006）、单核细胞（P = 0.008）、总T（CD3）淋巴细胞（P = 0.022）和辅助性T（CD4(+)）细胞（P = 0.003）均增加。白细胞与BMI百分位数、BMI或体脂百分比之间存在显著相关性。瘦体重和每单位瘦体重的最大摄氧量（VO₂peak）均与任何白细胞无显著相关性。体脂百分比和BMI百分位数与总T细胞（CD3）和/或辅助性T细胞（CD4(+)）呈正相关（P<0.05）。
一组23名超重儿童的大多数循环免疫细胞类型计数升高，提示存在低度全身炎症，这是肥胖大多数长期并发症的已知发病机制。我们的数据为避免或纠正儿童肥胖的重要性提供了额外的理论依据。
