Department of Pathophysiology, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Târgu Mureș, 540139 Târgu Mureș, Romania.
Doctoral School, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Târgu Mureș, 540139 Târgu Mureș, Romania.
Medicina (Kaunas). 2024 Sep 18;60(9):1523. doi: 10.3390/medicina60091523.
Obesity-associated chronic low-grade inflammation supports various systemic alterations. In this descriptive study, 122 apparently healthy adults aged 20 to 35 years were voluntarily included and classified based on body mass index (BMI) as normal-weight (NW), overweight (OW), and obese (OB). This study aims to characterize peripheral blood (PB) lymphocyte (Ly) phenotypes and investigate their correlations with body composition indices (BCIs) in healthy young adults. The following BCIs were measured: waist circumference, hip circumference, height, waist-to-hip ratio, waist-to-height ratio, total body fat mass, visceral fat level, weight, and BMI. White blood cell count (WBC), Ly absolute count, serum TNF-α, and IFN-γ were quantified. Ly subpopulations were analyzed as follows: total TLy (TTLy-CD45CD3), early activated TLy (EATLy-CD45369), total NKLy (TNKLy-CD45CD3CD56CD16), NK (low expression of CD56), NK (high expression of CD56), BLy (CD45CD3CD19), T helper Ly (ThLy-CD45CD3CD4), and T cytotoxic Ly (TcLy-CD45CD3CD8). Higher BMI has significantly higher WBC and BLy ( < 0.0001; = 0.0085). EATLy significantly decreased from NW to OB (3.10-NW, 1.10-OW, 0.85-OB, < 0.0001). Only EATLy exhibited significant negative correlations with all the BCIs. A significantly higher TNF-α was observed in the OW and OB groups compared to the NW group. IFN-γ increased linearly but nonsignificantly with BMI. TTLy showed a nonsignificant positive correlation with both IFN-γ and TNF-α, while EATLy showed a negative correlation, significant only for IFN-γ. NKLy subpopulations exhibited a consistent negative correlation with TNF-α, significant only for NK ( = 0.0423), and a nonsignificant consistent positive correlation with IFN-γ. A nonsignificant negative correlation between age and both TNKLy (r = -0.0927) and NK (r = -0.0893) cells was found, while a positive correlation was found with NK (r = 0.0583). In conclusion, the baseline immunological profile of PB is influenced by excessive adipose tissue in healthy young adults.
肥胖相关的慢性低度炎症支持各种全身改变。在这项描述性研究中，我们自愿纳入了 122 名年龄在 20 岁至 35 岁之间的明显健康成年人，并根据体重指数（BMI）分为正常体重（NW）、超重（OW）和肥胖（OB）。本研究旨在描述外周血（PB）淋巴细胞（Ly）表型，并研究其与健康年轻成年人的身体成分指数（BCI）的相关性。测量了以下 BCI：腰围、臀围、身高、腰臀比、腰高比、体脂肪量、内脏脂肪水平、体重和 BMI。量化了白细胞计数（WBC）、Ly 绝对计数、血清 TNF-α 和 IFN-γ。分析了 Ly 亚群，包括总 TLy（TTLy-CD45CD3）、早期激活 TLy（EATLy-CD45369）、总 NKLy（TNKLy-CD45CD3CD56CD16）、NK（CD56 低表达）、NK（CD56 高表达）、BLy（CD45CD3CD19）、ThLy（CD45CD3CD4）和 TcLy（CD45CD3CD8）。较高的 BMI 与较高的 WBC 和 BLy 显著相关（<0.0001；=0.0085）。EATLy 从 NW 到 OB 显著减少（3.10-NW，1.10-OW，0.85-OB，<0.0001）。只有 EATLy 与所有 BCI 呈显著负相关。OW 和 OB 组与 NW 组相比，TNF-α 显著升高。IFN-γ 随着 BMI 的增加呈线性增加，但无统计学意义。TTLy 与 IFN-γ 和 TNF-α 呈显著正相关，而 EATLy 与 IFN-γ 呈负相关，与 TNF-α 仅呈显著负相关。NKLy 亚群与 TNF-α 呈一致的负相关，与 IFN-γ 呈一致的正相关，但仅与 NK 呈显著相关（=0.0423）。我们发现年龄与 TNKLy（r=-0.0927）和 NK（r=-0.0893）细胞呈负相关，与 NK 呈正相关（r=0.0583）。综上所述，在健康的年轻成年人中，过量的脂肪组织会影响 PB 的基础免疫谱。
