Wray S, Wilkie D R
Department of Physiology, University of Liverpool, London, UK.
NMR Biomed. 1992 May-Jun;5(3):137-41. doi: 10.1002/nbm.1940050306.
Quantification of metabolites is a goal of many biomedical NMR studies. To obtain absolute measurements of metabolite concentrations is often both difficult and time-consuming. In this paper a method for determining metabolite concentrations directly is described and validated. It is applicable to studies of amphibian muscles, and with suitable precautions, to other isolated organs and tissues. The method is based upon using 14N NMR and nitrate-containing solutions to determine what fraction (F) of the sensitive volume of the RF coil is occupied by tissue. As the concentration of nitrate is known it can be used to calibrate other 14N metabolites in the tissue. Moreover, once F is determined, it can be used to calibrate metabolites in spectra from other nuclei e.g., 31P or 31C. All that is required is that a spectrum from a standard for that nucleus is obtained. Thus this method does not require any 'internal' (intrinsic to the tissue) standard, and is extremely quick and simple to use.
代谢物的定量分析是许多生物医学核磁共振研究的目标。获得代谢物浓度的绝对测量值往往既困难又耗时。本文描述并验证了一种直接测定代谢物浓度的方法。它适用于两栖类肌肉的研究，并且在采取适当预防措施的情况下，也适用于其他离体器官和组织。该方法基于使用14N核磁共振和含硝酸盐溶液来确定射频线圈敏感体积中被组织占据的比例（F）。由于硝酸盐的浓度是已知的，它可用于校准组织中的其他14N代谢物。此外，一旦确定了F，它可用于校准来自其他原子核（例如31P或31C）光谱中的代谢物。所需要的只是获得该原子核标准品的光谱。因此，该方法不需要任何“内部”（组织固有的）标准品，并且使用起来极其快速和简单。
