Buchli R, Boesiger P
Institute of Biomedical Engineering and Medical Informatics, University, Zurich, Switzerland.
Magn Reson Med. 1993 Nov;30(5):552-8. doi: 10.1002/mrm.1910300505.
In order to determine metabolite concentrations in human skeletal muscles by in vivo 31P MRS, different quantification methods were analyzed with regard to the accuracy and reproducibility of results and the simplicity of handling. Each quantification method comprised a calibration strategy and a localization technique. Extensive in vivo and in vitro tests showed that homonuclear phantom-based calibration strategies yielded significantly more accurate (lower systematic errors) and more reproducible (lower statistical errors) concentration estimates than heteronuclear strategies using internal water as a concentration standard. Additionally, the former strategies are easier to handle than the latter. Localization with the volume-selective sequence ISIS yielded slightly more reproducible results than localization by surface coil. We conclude that phosphorus metabolite concentrations are determined most accurately with phantom-based calibration strategies in combination with ISIS localization (measurement errors approximately 5-7%).
为了通过体内31P磁共振波谱法测定人体骨骼肌中的代谢物浓度，针对结果的准确性、可重复性以及操作的简便性，对不同的定量方法进行了分析。每种定量方法都包括一种校准策略和一种定位技术。广泛的体内和体外测试表明，与使用内部水作为浓度标准的异核策略相比，基于同核体模的校准策略能产生显著更准确（更低的系统误差）和更可重复（更低的统计误差）的浓度估计值。此外，前一种策略比后一种更易于操作。使用体积选择序列ISIS进行定位比通过表面线圈定位产生的结果稍更具可重复性。我们得出结论，结合ISIS定位（测量误差约为5 - 7%）的基于体模的校准策略能最准确地测定磷代谢物浓度。
