Wray S, Tofts P S
Biochim Biophys Acta. 1986 May 29;886(3):399-405. doi: 10.1016/0167-4889(86)90175-8.
Whilst in vivo NMR spectroscopy provides much useful biochemical information, a limitation to such studies has been the difficulty in quantitating the results to obtain absolute metabolite concentrations. We report here a simple direct method to obtain absolute metabolite concentrations when using in vivo NMR with radiofrequency surface coils. The method has been validated for nucleoside triphosphates in two tissues; rat brain and skeletal muscle. The results obtained are in close agreement with nucleoside triphosphate concentrations obtained using other methods. Precautions for the accurate application of the method are discussed. This method can be applied to other metabolites, coils and NMR nuclei.
虽然体内核磁共振波谱学提供了许多有用的生化信息，但此类研究的一个局限性在于难以对结果进行定量以获得绝对代谢物浓度。我们在此报告一种简单的直接方法，用于在使用射频表面线圈进行体内核磁共振时获得绝对代谢物浓度。该方法已在两种组织（大鼠脑和骨骼肌）中针对三磷酸核苷进行了验证。所获得的结果与使用其他方法获得的三磷酸核苷浓度密切一致。文中讨论了准确应用该方法的注意事项。此方法可应用于其他代谢物、线圈和核磁共振核。
