van Ree R, Voitenko V, van Leeuwen W A, Aalberse R C
Department of Allergy, Central Laboratory of The Netherlands Red Cross Blood Transfusion Service, Amsterdam.
Int Arch Allergy Immunol. 1992;98(2):97-104. doi: 10.1159/000236171.
Sera with IgE antibodies against grass pollen often contain IgE against vegetable foods. We investigated the role of the ubiquitous protein profilin in this cross-reactivity. Profilin was purified from Lolium perenne grass pollen by means of affinity purification with Sepharose-coupled poly(L-proline). This solid phase was also used as capturing agent for profilin from pollen and food extracts for application in a radioallergosorbent test. It was shown that profilin is an allergen in grass pollen and in a wide range of vegetable foods, like potato and celery. Within a grass-pollen-sensitive population, patients with IgE to vegetable foods have a high incidence of antibodies against profilin. IgE antibodies against grass pollen profilin were shown to be cross-reactive with respect to vegetable foods.
含有抗草花粉IgE抗体的血清通常也含有抗蔬菜食物的IgE。我们研究了普遍存在的肌动蛋白结合蛋白在这种交叉反应中的作用。通过用琼脂糖偶联的聚（L-脯氨酸）进行亲和纯化，从多年生黑麦草花粉中纯化出肌动蛋白结合蛋白。该固相也用作从花粉和食物提取物中捕获肌动蛋白结合蛋白的试剂，用于放射变应原吸附试验。结果表明，肌动蛋白结合蛋白是草花粉和多种蔬菜食物（如马铃薯和芹菜）中的一种过敏原。在对草花粉敏感的人群中，对蔬菜食物有IgE的患者中抗肌动蛋白结合蛋白抗体的发生率很高。已证明抗草花粉肌动蛋白结合蛋白的IgE抗体与蔬菜食物具有交叉反应性。
